烟酰胺类辅酶依赖型氧化还原酶的辅酶偏好性改造及其在合成生物学中的应用

刘美霞, 李强子, 孟冬冬, 魏欣蕾, 游淳

Protein engineering of nicotinamide coenzyme-dependent oxidoreductases for coenzyme preference and its application in synthetic biology

Meixia LIU, Qiangzi LI, Dongdong MENG, Xinlei WEI, Chun YOU

图5 由仿生辅酶NCD和NFCD与突变体酶苹果酸酶（ME）、D-乳酸脱氢酶（DLDH）和甲酸脱氢酶（FDH）组成的仿生辅酶正交体系

Fig. 5 Cofactor orthogonal system of NCD and NFCD with mutated malic enzyme (ME), D-lactate dehydrogenase (DLDH) and formate dehydrogenase (FDH)