DNA合成、组装与纠错技术研究进展

彭凯, 逯晓云, 程健, 刘莹, 江会锋, 郭晓贤

Advances in technologies for de novo DNA synthesis， assembly and error correction

Kai PENG, Xiaoyun LU, Jian CHENG, Ying LIU, Huifeng JIANG, Xiaoxian GUO

图2 TdT-dNTP 交联体介导的可逆终止用于寡核苷酸合成循环 [TdT (terminal deoxynucleotidyl transferase) is specifically conjugated with the desired dNTP to form TdT-dNTP conjugate before the reaction cycle. The conjugate is then incorporated into the 3' end of the primer in the polymerization reaction. The subsequent deprotection reaction removes TdT and unreacted TdT-dNTP conjugate to release the primer for next round of polymerization. The cycle is iterated to extend a primer by a defined sequence]

Fig. 2 The oligo synthesis cycle by TdT-dNTP conjugates mediated reversible termination