DNA合成、组装与纠错技术研究进展

彭凯, 逯晓云, 程健, 刘莹, 江会锋, 郭晓贤

Advances in technologies for de novo DNA synthesis， assembly and error correction

Kai PENG, Xiaoyun LU, Jian CHENG, Ying LIU, Huifeng JIANG, Xiaoxian GUO

图3 常见体外和体内DNA组装技术及流程 （Assembly of synthesized overlapping single-stranded oligos usually employ LCR and PCA based approaches. These assembly process turn oligos into longer double-stranded DNA constructs which can then be further assembled into longer fragments by Gibson assembly and Golden gate. In vivo assembly is typically achieved by feeding yeast cell with large fragments that have overlapping ends through transformation. The efficient DNA recombination machinery eventually string the fragments for assemblies that span hundreds of thousands or even millions of bases）

Fig. 3 Summary of general schemes of in vitro and in vivo DNA assembly