DNA合成、组装与纠错技术研究进展

彭凯, 逯晓云, 程健, 刘莹, 江会锋, 郭晓贤

Advances in technologies for de novo DNA synthesis， assembly and error correction

Kai PENG, Xiaoyun LU, Jian CHENG, Ying LIU, Huifeng JIANG, Xiaoxian GUO

图4 基于错配切割和错配结合的纠错策略 （Error rich DNA sequences are denatured and randomly re-hybridized to expose the errors introduced during oligo synthesis and subsequent assembly as bulging mismatches. The mismatches are then removed by error correction strategies using either mismatch-cleaving enzymes or mismatch-binding proteins. Fragments with fewer errors are recovered by overlap assembly extension PCR or filtering)

Fig. 4 Strategies for error-removal based on mismatch cleavage and mismatch binding