CRISPR基因编辑技术在微生物合成生物学领域的研究进展

李洋, 申晓林, 孙新晓, 袁其朋, 闫亚军, 王佳

Advances of CRISPR gene editing in microbial synthetic biology

Yang LI, Xiaolin SHEN, Xinxiao SUN, Qipeng YUAN, Yajun YAN, Jia WANG

图1 CRISPR/Cas9系统切割机制及修复机制图 [Cas9蛋白中蓝色代表识别DNA被识别的位点，橙色代表PAM序列，深紫色代表sgRNA上的识别序列；在HDR过程中，浅紫色代表同源序列，绿色代表进行替换的序列，橙色蛋白为RecE或Redα（负责供体DNA单链切割），蓝色蛋白为RecT或Redβ（负责黏性末端的保护以及和基因断裂位点的结合）；在NHEJ过程中，黄色蛋白表示Ku（负责保护断裂位点两侧），橙色蛋白为ligD（负责连接断裂位点）]

Fig. 1 The cleavage and repair mechanism of CRISPR/Cas9 systems （Blue represents the recognized site of DNA, orange represents PAM sequence, and dark purple represents the recognition sequence by sgRNA. During HDR, light purple represents homologous sequences, green represents substituted sequences, orange represents RecE or Red responsible for donor DNA single strand cutting, and blue represents RecT or Red responsible for protecting sticky ends and binding to the breaking sites of genes. During NHEJ, the yellow protein represents Ku responsible for protecting the fracture site, while the orange protein is ligD responsible for connecting to the fracture site)