CRISPR-Cas系统编辑丝状真菌的进展与挑战

肖晗, 刘宜欣

Progress and challenge of the CRISPR-Cas system in gene editing for filamentous fungi

Han XIAO, Yixin LIU

图1 CRISPR-Cas系统介导的丝状真菌基因编辑 [RNP—核糖核蛋白；BM-RNP—仿生矿化的RNP；PEG—聚乙二醇；AMT—根瘤农杆菌介导的原生质体转化；DSB—DNA双链缺口；NHEJ—非同源末端连接；HR—同源重组；APOBEC1—载脂蛋白B mRNA编辑酶（胞嘧啶脱氨酶）；VPR—含有三个转录激活结构域的转录激活因子；CRISPRa—CRISPR介导的基因转录激活系统]

Fig. 1 CRISPR-Cas system assisted gene editing for filamentous fungi （RNP—ribonucleoprotein; BM-RNP—biomimetic mineralized RNP; PEG—polyethylene glycerol; AMT—agrobacterium-mediated transformation; DSB—double stranded break; NHEJ—non-homologous end joining; HR—homologous recombination; APOBEC1—apolipoprotein B mRNA editing enzyme; VPR—VP64-p65-Rta, a tripartite transcriptional activator domain; CRISPRa—CRISPR activation）