CRISPR-Cas系统编辑丝状真菌的进展与挑战

肖晗, 刘宜欣

Progress and challenge of the CRISPR-Cas system in gene editing for filamentous fungi

Han XIAO, Yixin LIU

图2 CRISPR-Cas系统在丝状真菌中体内表达策略 （针对Cas蛋白的体内表达，有密码子优化，采用组成型启动子驱动蛋白表达和在Cas基因5' 端引入内含子等策略。针对gRNA的体内表达，有用RNA聚合酶II型启动子联合具有自我剪切功能的核酶表达gRNA、RNA聚合酶III型启动子表达gRNA、RNA聚合酶III型启动子联合具有自我剪切功能的核酶表达gRNA等策略）

Fig. 2 Strategies for in vivo expression of the CRISPR-Cas system in filamentous fungi （For in vivo expression of Cas protein, strategies include codon-optimization, adopting constitutive promoter for driving the expression of Cas protein, and incorporating intron sequence at the 5' end of cas gene. For in vivo expression of gRNA, strategies include adopting polymerase II promoter together with the self-cleaving ribozymes for gRNA expression, adopting polymerase III promoter for gRNA expression, and adopting polymerase III promoter together with the self-cleaving ribozyme for gRNA expression）