CRISPR-Cas系统编辑丝状真菌的进展与挑战

肖晗, 刘宜欣

Progress and challenge of the CRISPR-Cas system in gene editing for filamentous fungi

Han XIAO, Yixin LIU

图3 同源重组供体的设计 （线性双链DNA供体，其两侧同源臂长39 bp, 距离CRISPR-Cas系统产生的DNA双链缺口5 kb以内；环状DNA供体，两侧同源臂长约0.5～2 kb，中间往往包含筛选标记；单链寡核苷酸，供体中突变位点和PAM之间距离不超过7 bp）

Fig. 3 Design of homologous recombination donor （For linearized DNA, it is flanked by 39 bp HR arms, which are within 5 kb from DSB as generated by CRIPSR-Cas. For circular DNA, it is flanked by HR arms with the size from 0.5 to 2 kb, and a selective marker is usually contained in the middle. For oligo, the intended mutation and PAM sequence are interspaced by no more than 7 bp）