创制非天然辅酶偏好型甲醇脱氢酶
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王俊婷, 郭潇佳, 李青, 万里, 赵宗保
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Creation of non-natural cofactor-dependent methanol dehydrogenase
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Junting WANG, Xiaojia GUO, Qing LI, Li WAN, Zongbao ZHAO
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表1 MDH及突变体催化甲醇氧化的动力学数据
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Tab. 1 Kinetic data of methanol oxidation catalyzed by MDH variants
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| Enzyme | NAD | | NCD | NCD preference [(kcat/Km)NCD/ (kcat/Km)NAD] |
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| Km/（μmol/L） | kcat/s-1 | kcat/Km /[L/(mol·s)] | | Km/(μmol/L) | kcat/s-1 | kcat/Km /[L/(mol·s)] |
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| WT | 25.5±4.4 | 6.1×10-2±0.1×10-2 | 2.4×103 | | 328.6±35.8 | 2.5×10-2±0.1×10-2 | 76.1 | 3.2×10-2 | | 3D2 | 6.6±0.5 | 1.3×10-2±0.0 | 2.0×103 | | 115.6±11.4 | 2.0×10-2±0.0 | 173.0 | 8.7×10-2 | | 3F1 | 121.5±24.4 | 1.0×10-3±0.0 | 8.2 | | 77.2±11.3 | 6.0×10-3±0.0 | 77.7 | 9.5 | | 3-5 | 5.3±2.5 | 1.0×10-3±0.0 | 188.7 | | 47.1±7.7 | 6.0×10-3±0.0 | 127.4 | 0.7 | | 3-1 | 728.1±95.4 | 9.0×10-3±0.0 | 12.4 | | 44.3±6.6 | 1.3×10-2±0.0 | 293.5 | 23.7 | | 9D1 | 1 018.2±257.1 | 2.0×10-3±0.0 | 2.0 | | 33.8±10.2 | 2.9×10-2±0.0 | 858.0 | 4.3×102 |
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