虫生真菌非核糖体多肽活性产物生物合成潜力预测

张礼文, MOLNÁR István, 徐玉泉

Potential biosynthesis of nonribosomal peptides by hypocrealean entomopathogenic fungi

Liwen ZHANG, István MOLNÀR, Yuquan XU

图1 肉座菌目虫生真菌多模块非核糖体多肽合成酶的相似性网络图 ［其中每个节点代表一个腺苷酰化结构域，连接线粗细与相似性成正比。节点颜色显示非核糖体多肽合成酶的类型，形状表示肉座菌目虫生真菌的种类（科），已知功能的结构域用箭头表示。灰色边框中显示来自同一酶的腺苷酰化结构域的分支（如DtxS1），或由密切相关的结构域分支（如52～64）形成的超级分支（详见正文）。底色显示预测的产物类型。腺苷酰化结构域的亚分支用数字字母组合表示，例如52a］

Fig. 1 Network for the subgroups of multi-modular NRPSs in Hypocrealean Entomopathogenic fungi (Nodes in the network represent A domains, and the line boldness is proportional to the identity. The nodes are colored according to the domain composition of NRPS. Referenced A domains from voucher NRPSs with known products are indicated by arrowheads. The predicted products/functions of the enzymes are listed below the clades. Boxed groups show A domains from the group of enzymes that also contain DtxS1, or superclades that are formed by closely related A domain clades such as clades #52～64 (see text for details). Sub-clades of A domains are indicated with a number-letter combination, for example 52a. NRPSs of the BGCs that contain the voucher A domains include: #27a～32a, destruxin synthetase DtxS1[9]; #32b, cyclosporine synthetase[29]; #33～39, serinocyclin synthetase[13-14]; #44～45, ACVS: N-(5-amino-5-carboxypentanoyl)-L-cysteinyl-D-valine synthetase in penicillin/cephalosporin biosynthesis; #46～51, Tex1: a peptaibol synthetase from Trichoderma virens[30]; #75～76, beauvericin and bassianolide synthetase[11-12].)