虫生真菌非核糖体多肽活性产物生物合成潜力预测

张礼文, MOLNÁR István, 徐玉泉

Potential biosynthesis of nonribosomal peptides by hypocrealean entomopathogenic fungi

Liwen ZHANG, István MOLNÀR, Yuquan XU

图3 肉座菌目虫生真菌单模块和双模块非核糖体多肽合成酶的相似性网络图 ［其中每个节点代表一个腺苷酰化结构域，连接线粗细与相似性成正比。节点颜色显示非核糖体多肽合成酶的类型，形状表示肉座菌目虫生真菌的种类（科），已知功能的结构域用箭头表示。绿色阴影标注产物未知的非核糖体多肽合成酶腺苷酰化结构域。括号中的数字表示腺苷酰化结构域在标签非核糖体多肽合成酶中的位置，例如（1/3）表示该结构域是一个具有3个模块的非核糖体多肽合成酶的第1个腺苷酰化结构域。AAR—L-氨基己二酸半醛脱氢酶［24］；LpsC和LpsB—D-麦角酰肽合成酶亚基1和2，参与麦角生物碱的生物合成［32］；ChNPS10—参与真菌形态发育［33-34］；NPS2/SidC［35-36］和SidI［37］—胞内铁载体合成酶；SidN［38］和NPS6［39］：胞外铁载体合成酶］

Fig. 3 Overview of the A domain distance network for monomodular, bimodular or siderophore-like NRPSs in Hypocrealean entomopathogenic fungal species (Nodes in the network represent A domains, and the line boldness is proportional to the identity. Nodes are colored according to the domain composition of NRPS. Referenced A domains from voucher NRPSs with known products are indicated by arrowheads. The predicted products/functions of the enzymes are listed below the clades. NRPSs that contain the voucher A domains include: AAR, L-aminoadipate-semialdehyde dehydrogenase[24]; LpsC and LpsB: D-lysergyl-peptide synthetase subunit 1 and 2, respectively, involved in ergot alkaloid biosynthesis[32]; ChNPS10[33-34]: an NRPS-like protein involved in morphological development; NPS2/SidC[35-36] and SidI[37]: synthetases for intracellular siderophores; SidN[38] and NPS6[39]: synthetases for extracellular siderophores. Numbers in parentheses indicate the position of the A domain in the voucher NRPS, for example (1/3) indicates the first A domain out of the three housed in the NRPS.)