利用异源表达挖掘纤维堆囊菌So0157-2的新型天然产物

周海波, 申琪瑶, 陈汉娜, 王宗杰, 李越中, 张友明, 卞小莹

Genome mining for novel natural products in Sorangium cellulosum So0157-2 by heterologous expression

ZHOU Haibo, SHEN Qiyao, CHEN Hanna, WANG Zongjie, LI Yuezhong, ZHANG Youming, BIAN Xiaoying

表1 本研究所用的菌株和质粒

Tab. 1 Strains and plasmids used in this study

Strains/Plasmids Description Sources Strains [21] Escherichia coli GB05-dir GB2005, araC-PBAD-ETgA; recE, recT, redγ, and recA regulated by arabinose-induced promoter are inserted at ybcC locus E. coli GB05-red GB2005, araC-PBAD-αβγA; redα, redβ, redγ, and recA regulated by arabinose-induced promoter are integrated at ybcC locus [38] S. cellulosum So0157-2 wild type harboring BGC18 [7-8] S. brevitalea DSM 7029 Burkholderiales strain DSM 7029, [Polyangium] brachysporum DSM 7029 (K481-B101; ATCC 53080) [35-36,39] DSM7029:Pkm-BGC18 Pkm-BGC18 was inserted into genome of Schlegelella brevitalea DSM 7029, kmR This work Plasmids p15A-cm-tetR-tetO-hyg-ccdB direct cloning vector, p15A replicon, containing a tetracycline inducible promoter PtetO, cmR/hygR [24] pR6K-oriT-TnpA-IR-km R6K replicon, containing MycoMar transposase gene (tnpA) and conjugation element oriT, kmR [24] p15A-cm-BGC18 BGC18 was cloned into cloning vector, cmR This work p15A-oriT-IR-Pkm-BGC18 Expression vector of BGC18 with Pkm was inserted into upstream of the first core gene of BGC18, containing transposon elements oriT-tnpA-IR, kmR This work