利用异源表达挖掘纤维堆囊菌So0157-2的新型天然产物

周海波, 申琪瑶, 陈汉娜, 王宗杰, 李越中, 张友明, 卞小莹

Genome mining for novel natural products in Sorangium cellulosum So0157-2 by heterologous expression

ZHOU Haibo, SHEN Qiyao, CHEN Hanna, WANG Zongjie, LI Yuezhong, ZHANG Youming, BIAN Xiaoying

表2 本研究所用的引物

Tab. 2 Primers used in this study

Primers Sequences (5′-3′) BGC18-HAF cagtatggccgatcggggtgtcagcggtcaacacgcgagctcgctcgctctgtggcggcgctctcatggtgcAACGCTCTCTACTAGAGTCA BGC18-HAR gacgtgaccgggtatcgctaggtccacccaccaggagtccggccagggatccggagagtgagagttcaacgcGGGTCTTAAGACGTCGATATCT IR-Pkm-C18-HAF1 gcctgcgatcgtaccattacgtatttttgcgcgagcggaacggtatgcagGCTGATCTTCAGATCCTCTAC IR-Pkm-C18-HAR1 tcgagccgcaaggcgcattcttgctccagagagagcggcatagtacccaacctcctTCAGAAGAACTCGTCAAGAAG detect-C18 in 7029-F1 GATGGGTTATCAGGACTACGC detect-C18 in 7029-R1 CGAGGAGCCTGTAGAACGCGT detect-C18 in 7029-F2 TCGACGATCAGGTGAAGATCCA detect-C18 in 7029-R2 AGCTCTCCATAGGTGAGCGAA detect-C18 in 7029-F3 TGAAGATCCGCGGCTATCGCA detect-C18 in 7029-R3 CGGGAACTGGAACAGGTCCAT detect-C18 in 7029-F4 TTCTTCGTCAACGCCGCGCC detect-C18 in 7029-R4 GCGATAGGCGTGCACCGTGC detect-C18 in 7029-F5 TCACGCTGCCGCAAGCACTC detect-C18 in 7029-R5 GCTCTGCGAAGGACGTCCTC