定向进化在蛋白质工程中的应用研究进展

祁延萍, 朱晋, 张凯, 刘彤, 王雅婕

Recent development of directed evolution in protein engineering

QI Yanping, ZHU Jin, ZHANG Kai, LIU Tong, WANG Yajie

图2 基于CRISPR的体内突变方法［7］ a—基于CRISPR-Cas9同源重组的突变；b—基于nCas9和DNA聚合酶I的突变；c—基于nCas9和碱基编辑器的突变

Fig. 2 CRISPR-assisted in vivo mutagenesis[7] a—CRISPR-Cas9-HDR; b—Random mutagenesis induced by nCas9-E. coli DNA PolI (error-prone) hybrid proteins; c—Gene mutangenesis caused by nCas9-deaminase hybrid proteins