CRISPR/Cas9系统在基因组编辑中的优化与发展

滕小龙, 史硕博

Optimization and development of CRISPR/Cas9 systems for genome editing

TENG Xiaolong, SHI Shuobo

图1 CRISPR/Cas9系统基因编辑原理 ［左侧为非同源末端连接修复（NHEJ）介导的片段删除/片段插入原理图；中间为同源重组修复（HDR）介导的基因编辑原理图；右侧为微同源末端连接（MMEJ）介导的基因编辑原理图］

Fig. 1 Gene editing mechanism of CRISPR/Cas9 systems (NHEJ-mediated fragment deletion/insertion, HDR-mediated gene editing and MMEJ-mediated gene editing are shown diagrammatically from left and middle to right)