重组胶原蛋白表达体系研究进展
Research progress on recombinant collagen expression system
P—脯氨酸残基；K—赖氨酸残基；P4H—脯氨酰4-羟化酶；LH—赖氨酰羟化酶；P3H1—脯氨酰3-羟化酶；HSP47—热休克蛋白47；PDI—二硫异构酶；FKPB65—免疫亲蛋白；PNP—原胶原N端酶；PCP—原胶原C端酶（首先胶原蛋白 α 链经内质网中P4H酶以及LH酶的作用下实现羟基化，随后，3条胶原蛋白α单链的C端前肽在内质网膜凝集素样分子伴侣、钙联蛋白和内质网氧化还原酶PDI的协同作用下形成二硫键。HSP47能有效防止前胶原蛋白局部展开和聚集形成。最后，前胶原蛋白经高尔基体运送至细胞质基质利用N端酶与C端酶将多余N端与C端进行切除，形成完整的胶原蛋白结构）
P—proline residue; K—lysine residue; P4H—prolyl 4-hydroxylase; LH—lysyl hydroxylase; P3H1—prolyl 3-hydroxylase; HSP47—heat shock protein 47; PDI—disulfide isomerase; FKPB65—immunophilic proteins; PNP—procollagen N protease; PCP—procollagen C protease (Firstly, collagen genes form collagen α-chains through transcription and translation, and secondly, under the action of P4H enzymes and LH enzymes in the endoplasmic reticulum, collagen α-chain is hydroxylated. Subsequently, three collagen α single-stranded C-terminal propeptides form disulfide bonds under the synergistic action of endoplasmic reticulum lectin-like chaperones, calcepiprotein, and endoplasmic reticulum oxidoreductase PDI. HSP47 effectively prevents the formation of local expansion and aggregation of pre-collagen. Finally, the cytoplasmic matrix of the procollagen is transported by the Golgi apparatus and the excess N-terminal and C-terminus of the precollagen are excised by N-terminal enzymes and C-terminal enzymes to form a complete collagen structure.)
