紫杉醇生物合成机制研究进展

刘晓楠, 李静, 祝晓熙, 徐子硕, 齐健, 江会锋

Research advances on paclitaxel biosynthesis

LIU Xiaonan, LI Jing, ZHU Xiaoxi, XU Zishuo, QI Jian, JIANG Huifeng

图2 紫杉醇生物合成途径解析 ［模块1代表巴卡亭Ⅲ生物合成途径推测1（蓝色）和巴卡亭Ⅲ生物合成途径推测2（黄色）。模块2（绿色）表示β-苯丙烯醇侧链生物合成途径。模块3（紫色）代表紫杉醇生物合成途径。TS—紫杉二烯合成酶；T5αOH—紫杉二烯-5α-羟化酶；TAT—紫杉二烯-5α-醇-O-乙酰基转移酶；T10βOH—紫杉烷-10β-羟化酶；T13αOH—紫杉烷-13α-羟化酶；T2αOH—紫杉烷-2α-羟化酶；T9αOH—紫杉烷-9α-羟化酶；T7βOH—紫杉烷-7β-羟化酶；T1βOH—紫杉烷-1β-羟化酶；TBT—紫杉烷-2α-O-苯甲酰转移酶；DBAT—10-去乙酰巴卡亭Ⅲ10-O-乙酰转移酶；TOT—紫杉烷环氧化酶；BAPT—巴卡亭Ⅲ-3-氨基，13-苯丙酰转移酶；T2′αOH—紫杉烷-2′α-羟化酶；DBTNBT—3′-N-去苯甲酰-2′-脱氧紫杉醇-N-苯酰转移酶；PAM—苯丙氨酸氨基变位酶；PCL—β-苯丙氨酸辅酶A连接酶］

Fig. 2 Recently discovered pathways for paclitaxel biosynthesis [Module 1 representing speculated baccatin Ⅲ biosynthetic pathway 1 (bule) and speculated baccatin Ⅲ biosynthetic pathway 2 (yellow). Module 2 (green) representing the β-phenylalanoyl side chain pathway. Module 3 (purple) representing paclitaxel biosynthetic pathway. TS—taxadiene synthase; T5αOH—taxane 5α-hydroxylase; TAT—taxadiene-5α-ol-O-acetyl transferase; T10βOH—taxane 10β-hydroxylase; T13αOH—taxane 13α- hydroxylase; T2αOH—taxane 2α-hydroxylase; T9αOH—taxane 9α-hydroxylase; T7βOH—taxane 7β-hydroxylase; T1βOH—taxane 1β-hydroxylase; TBT—taxane 2α-O-benzoyltransferase; DBAT—10-deacetyl baccatin Ⅲ-10-O-acetyltransferase; TOT—taxane oxetanase; BAPT—baccatin Ⅲ-3-amino, 13-phenylpropanoyltransferase; T2′αOH—taxane 2′α-hydroxylase; DBTNBT—3′-N-debenzoyl-2′-deoxytaxol-N-benzoyltransferase; PAM—phenylalanine aminomutase; PCL—β-phenylalanine coenzyme A ligase.]