紫杉醇生物合成机制研究进展

刘晓楠, 李静, 祝晓熙, 徐子硕, 齐健, 江会锋

Research advances on paclitaxel biosynthesis

LIU Xiaonan, LI Jing, ZHU Xiaoxi, XU Zishuo, QI Jian, JIANG Huifeng

表1 已知的紫杉醇生物合成酶及参考文献

Table 1 Identified paclitaxel biosynthetic enzymes

酶名称 简写 登录号 参考文献 1 Geranylgeranyl diphosphate synthase GGPPS AF081514 [14] 2 Taxadiene synthase TS AY364469 [15] 3 Taxadiene-5α-hydroxylase T5αOH AY289209 [16] 4 Taxadiene-13α-hydroxylase T13αOH AY056019 [17] 5 Taxadiene-5α-ol-O-acetyl transferase TAT AF190130 [18] 6 Taxadiene-10β-hydroxylase T10βOH AF318211/AY563635 [19-20] 7 Taxadiene-7β-hydroxylase T7βOH AY307951 [21] 8 Taxadiene-2α-hydroxylase T2αOH AY518383 [22] 9 Taxane-2α-O-benzoyl transferase TBT AF297618 [23] 10 10-deacetylbaccatin Ⅲ-10-O-acetyl transferase DBAT AF193765 [23] 11 Phenylalanine aminomutase PAM AY582743 [24] 12 β-phenylalanoyl-CoA ligase PCL KM593667 [25] 13 Baccatin Ⅲ: 3-amino, 13-phenylpropanoyltransferase BAPT AY082804 [25] 14 Taxane-2′α-hydroxylase T2′αOH KP178208 [26] 15 N-benzoyl transferase DBTNBT AF466397 [27] 16 Taxadiene-14β-hydroxylase T14βOH AY188177 [28] 17 Cytochrome P450 reductase TcCPR AY571340 [29] 18 C4β-C20 epoxidase [12] 19 Taxane 1β-hydroxylase T1βOH [12] 20 Taxane 9α-hydroxylase T9αOH [12] 21 Taxane 9α-dioxygenase [12] 22 Phenylalanine-CoA ligase PCL [12] 23 Taxane oxetanase TOT [13] 24 Taxane 9α-hydroxylase T9αH1 [13] 25 Taxane 9α-hydroxylase CYP725A37 PP197199/PP197200 [30] 26 Taxane oxetanase CYP725A55 PP197201 [30] 27 Acyltransferase AT5 PP197202 [30]