中药药效成分群的合成生物学研究进展

查文龙, 卜兰, 訾佳辰

Advances in synthetic biology for producing potent pharmaceutical ingredients of traditional Chinese medicine

Wenlong ZHA, Lan BU, Jiachen ZI

图1 檀香挥发油成分群的生物合成途径 （DXS—1-脱氧-D-木酮糖-5-磷酸合酶；DXR—1-脱氧-D-木酮糖-5-磷酸还原异构酶；MCT—2-C-甲基-D-赤藻醇-4-磷酸胞苷酰转移酶；CMK—4-（5′-焦磷酸胞苷）-2-C-甲基-D-赤藓醇激酶；MCS—2-C-甲基-D-赤藓醇-2，4-环焦磷酸合成酶；HDS—1-羟基-2-甲基-2-丁烯-4-焦磷酸合成酶；HDR—1-羟基-2-甲基-2-丁烯-4-焦磷酸还原酶；IDI—异戊烯基焦磷酸异构酶；AACT—乙酰辅酶A酰基转移酶；HMGS—3-羟基-3-甲基戊二酰辅酶A合成酶；HMGR—3-羟基-3-甲基戊二酰辅酶A还原酶；MK—甲羟戊酸激酶；PMK—磷酸甲羟戊酸激酶；MPD—焦磷酸甲羟戊酸脱羧酶）

Fig. 1 Biosynthetic pathway for the production of sandalwood oil (DXS—1-deoxy-D-xylulose 5-phosphate synthase; DXR—1-deoxy-D-lxylulose 5-phosphate reductoisomerase; MCT—2-C-methyl-D-erythritol-4-phosphate cytidyltransferase; CMK—4-(cytidine 5′-diphospho)-2-C-methyl-D-erythritol kinase; MCS—2-C-methyl-D-erythritol 2,4-diphosphate synthase; HDS—1-hydroxy-2-methyl-2-E-butenyl-4-diphosphate synthase; HDR—1-hydroxy-2-methyl-2-butenyl-4-diphosphate reductase; IDI—isopentenyl pyrophosphate isomerase; AACT—acetyl-coenzyme A acetyltransferase; HMGS—3-hydroxy-3-methylglutaryl-CoA synthase; HMGR—3-hydroxy-3-methylglutaryl-CoA reductase; MK—mevalonate kinase; PMK—phosphate mevalonate kinase; MPD—mevalonate pyrophosphate decarboxylase)