一碳化合物生物利用的合成生物学研究进展

李健, 陈云, 刘海艳, 谭在高

Advances in the biological utilization of one-carbon compounds

LI Jian, CHEN Yun, LIU Haiyan, TAN Zaigao

图1 天然的C1化合物代谢途径 （虚线表示多步反应。Ser，丝氨酸；Gly，甘氨酸；PEP，磷酸烯醇式丙酮酸；DC，二羧酸；4-HB，4-羟基丁酸；3-HP，3-羟基丙酸；Malyl-CoA，苹果酰CoA；H6P，己酮糖-6-磷酸；Xu5P，木酮糖-5-磷酸；DHA，二羟丙酮；RuBP，核酮糖-1，5-二磷酸；PGA，3-磷酸甘油酸；α-KG，α-酮戊二酸；SHMT，丝氨酸羟甲基转移酶；Ppc，PEP羧化酶；PFR，丙酮酸-铁氧还原蛋白氧化还原酶；Pcc，丙酰CoA羧化酶；ACC，乙酰CoA羧化酶；HPS，己酮糖-6-磷酸合酶；DAS，二羟丙酮合酶；RuBisCo，核酮糖-1，5-二磷酸羧化酶；IDH，异柠檬酸脱氢酶；KOR，α-酮戊二酸合酶；ACL，ATP依赖的柠檬酸裂解酶。）

Fig. 1 Natural metabolic pathways of one-carbon compounds (Dotted lines indicate multi-step reactions. Ser, serine; Gly, glycine; PEP, phosphoenolpyruvate; DC, dicarboxylate; 4-HB, 4-hydroxybutanoate; 3-HP, 3-hydroxypropanoate; H6P, hexulose 6-phosphate; Xu5P, xylulose 5-phosphate; DHA, dihydroxyacetone; RuBP, ribulose-1,5-bisphosphate; PGA, 3-phosphoglycerate; α-KG, α-ketoglutarate; SHMT, serine hydroxymethyltransferase; Ppc, PEP carboxylase; PFR, pyruvate-ferredoxin oxidoreductase; Pcc, propionyl-CoA carboxylase; ACC, acetyl-CoA carboxylase; HPS, hexulose-6-phosphate synthase; DAS, dihydroxyacetone synthase; RuBisCo, ribulose-1, 5-bisphosphate carboxylase; IDH, isocitrate dehydrogenase; KOR, α-ketoglutarate synthase; ACL, ATP-depentent citrate lyase.)