一碳化合物生物利用的合成生物学研究进展

李健, 陈云, 刘海艳, 谭在高

Advances in the biological utilization of one-carbon compounds

LI Jian, CHEN Yun, LIU Haiyan, TAN Zaigao

图2 人工设计的C1代谢途径 （（a）人工固碳途径。虚线表示多步反应。PEP，磷酸烯醇式丙酮酸；3-HP，3-羟基丙酸；Ppc，PEP羧化酶；Ccl，柠檬酰辅酶A裂解酶；Pcc，丙酰CoA羧化酶；Ccr，巴豆酰辅酶A羧化酶；Mcl，苹果酰CoA裂解酶；CA，碳酸酐酶。（b）人工甲醇同化途径。虚线表示多步反应。MeOH，甲醇；FLAD，甲醛；GALD，羟乙醛；H6P，己糖-6-磷酸；F6P，果糖-6-磷酸；AcP，乙酰磷酸；AcCoA，乙酰CoA；DHAP，磷酸二羟丙酮；Eu1P，赤酰酮糖-1-磷酸；Eu4P，赤酰酮糖-4-磷酸；E4P，赤酰糖-4-磷酸；GAP，甘油醛-3-磷酸；Ru5P，核酮糖-5-磷酸；Xu5P，木酮糖-5-磷酸；R5P，核糖-5-磷酸；S7P，景天庚酮糖-7-磷酸；FDP，果糖-1，6-二磷酸； PYC，丙酮酸羧化酶；HACL，2-羟基酰-CoA裂解酶；GLAS，羟乙醛合酶；HAS，己糖-6-磷酸醛缩酶；PFK，磷酸解酮酶；EPS， 赤酰酮糖-1-磷酸合酶。）

Fig. 2 Artificial metabolic pathways of one-carbon compounds ((A) Artificial carbon fixation pathways. Dotted lines indicate multi-step reactions. PEP, phosphoenolpyruvate; 3-HP, 3-hydroxypropanoate; Ppc, PEP carboxylase; Ccl, citramalyl-CoA lyase; Pcc, propionyl-CoA carboxylase; Ccr, crotonyl-CoA carboxylase; Mcl, malyl-CoA lyase; CA, carbonic anhydras. (B) Artificial methanol assimilation pathways. Dotted lines indicate multi-step reactions. MeOH, methanol; FLAD, Formaldehyde; GALD, glycolaldehyde; H6P, hexose-6-phosphate; F6P, fructose 6-phosphate; AcP, acetyl-phosphate; AcCoA, acetyl-CoA; DHAP, dihydroxyacetone phosphate; Eu1P, erythrulose 1-phosphate; Eu4P, erythrulose 4-phosphate; E4P, erythrose 4-phosphate; GAP, glyceraldehyde 3-phosphate; Ru5P, ribulose 5-phosphate; Xu5P, xylulose 5-phosphate; R5P, ribose 5-phosphate; S7P, sedoheptulose 7-phosphate; FDP, fructose-1,6-bisphosphate; PYC, pyruvate carboxylase; HACL, 2-hydroxyacyl-CoA lyase; EPS, erythrulose 1-phosphate synthase.)