固氮合成生物学研究进展

李超, 张焕, 杨军, 王二涛

Research advances in nitrogen fixation synthetic biology

LI Chao, ZHANG Huan, YANG Jun, WANG Ertao

图1 设计或改进生物固氮的策略 （I：改良固氮菌，增强固氮酶活性，提高铵的外排效率；II：增强作物根际招募有益固氮菌并调控固氮菌固氮活动的能力；III：解析结瘤机制，实现非豆科植物共生固氮；IV：在植物细胞器表达固氮酶，实现植物自主固氮）

Fig. 1 Main approaches to engineer or improve biological nitrogen fixation (I: Engineer nitrogen-fixing bacteria to enhance nitrogenase activity and optimize ammonium excretion efficiency; II: Strengthen crops' ability to recruit beneficial diazotrophs in the rhizosphere and regulate their nitrogen-fixing activity; III: Decipher nodulation mechanisms to achieve symbiotic nitrogen fixation in non-leguminous plants; IV: Express nitrogenase in plant organelles to enable autonomous nitrogen fixation in crops.)