活细胞记录器在细胞谱系追踪中的应用和前景

姜百翼, 钱珑

Application and prospect of live cell DNA-based molecular recorders in cell lineage tracing

JIANG Baiyi, QIAN Long

图1 理想的分子记录器［42-44］和Cas9分子记录器的代表性研究［41-42，45-49］ [Rosa26 Col1a1：基因组上landing pad位点，用于外源基因的定点整合。R26， pU6， CAG， EF1A：组成型启动子。TetO：诱导型启动子，由rtTA激活。M2-rtTA：诱导系统，由诱导剂doxycycline（dox） 激活]

Fig. 1 Ideal molecular recorders [42-44] and representative studies of Cas9-based molecular recorders[41-42,45-49] [Rosa26, Col1a1: landing pad sites on genome, for site-specific integration of exogenous genes. R26, pU6, CAG, EFIA: constitutive promoters. TetO: inducible promoter, activated by rtTA. M2-rtTA: inducible system, activated by doxycycline(dox) inducer]