活细胞记录器在细胞谱系追踪中的应用和前景

姜百翼, 钱珑

Application and prospect of live cell DNA-based molecular recorders in cell lineage tracing

JIANG Baiyi, QIAN Long

图3 三种新型分子记录器的工作原理［61-64］ (pegRNA—先导编辑引导RNA；TRE—在DOX存在时可被rtTA激活的启动子；HsAID—碱基编辑器；iSceI—DNA结合蛋白；rtTA—诱导系统)

Fig. 3 Working principles for three novel molecular recorders[61-64] (pegRNA—prime editing guide RNA;TRE—a promoter that can be activated by rtTA in the presence of DOX; HsAID—base-editor;iSceI—DNA binding protein; rtTA—inducible system)