合成生物学在干细胞早期胚胎发育模型中的应用

杨莹, 李霞, 刘立中

Applications of synthetic biology to stem-cell-derived modeling of early embryonic development

YANG Ying, LI Xia, LIU Lizhong

图3 光遗传工具在发育生物学中的应用 （通过光-氧-电压感应结构域介导的二聚化调控细胞内酪氨酸激酶活性，进而引发SMAD1/5的磷酸化，并促使其进入细胞核以启动BMP下游基因的表达。光遗传激活的Shroom3通过在顶端连接处招募ROCK，驱动细胞顶端收缩）

Fig. 3 Applications of optogenetic tools in developmental biology (Dimerization mediated by the light-oxygen-voltage (LOV) sensing domain regulates intracellular tyrosine kinase activity, leading to SMAD1/5 phosphorylation and their translocation into the nucleus to initiate downstream BMP target gene expression. Optogenetically activated Shroom3 drives apical constriction through ROCK recruitment at apical junctions.)