中性核心母乳寡糖生物合成的研究进展和发展趋势 |
| 刘丹, 王建宇, 江正强 |
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Research progress and development trends in the biosynthesis of neutral core human milk oligosaccharides |
| LIU Dan, WANG Jianyu, JIANG Zhengqiang |
| 图2 大肠杆菌合成LNT Ⅱ、LNnT和LNT路径图 (ptsG—编码葡萄糖特异性PTS转运系统IIBC组分基因;glk—编码己糖激酶基因;glpF—编码甘油摄取促进蛋白基因;gloA—编码乳酸谷胱甘肽裂合酶基因;gloB—编码羟酰谷胱甘肽水解酶基因;nagE—编码N-乙酰葡萄糖胺(GlcNAc)PTS转运系统IIABC组分基因;pfkA—编码6-磷酸果糖激酶基因;fbp—编码1,6-二磷酸果糖磷酸化激酶基因;zwf—编码葡萄糖-6-磷酸脱氢酶基因;pgi—编码葡萄糖-6-磷酸异构酶基因;pgm—编码磷酸葡萄糖变位酶基因;agp—编码双功能葡萄糖-1-磷酸酶/肌醇磷酸酶基因;gcd—编码葡萄糖脱氢酶基因;galU—编码UTP-葡萄糖-1-磷酸尿苷酰转移酶基因;galE—编码UDP-葡萄糖差向异构酶基因;otsA—编码α,α-海藻糖-6-磷酸合酶基因;ugd—编码UDP-葡萄糖-6-脱氢酶基因;galK—编码半乳糖激酶基因;galT—编码半乳糖-1-磷酸尿苷酰转移酶基因;glmS—编码葡糖胺-6-磷酸合酶基因;glmM—编码磷酸葡糖胺变位酶基因;glmU—编码N-乙酰葡糖胺-1-磷酸尿酸基转移酶/氨基葡糖-1-磷酸乙酰基转移酶基因;nagB—编码6-磷酸氨基葡萄糖脱氨酶基因;nagA—编码N-乙酰氨基葡萄糖-6-磷酸脱乙酰酶基因;murA—编码UDP-N-乙酰氨基葡萄糖-1-羧基乙烯基转移酶基因;wecB—编码UDP-N-乙酰氨基葡萄糖-2-差向异构酶基因;lacZ—编码β-半乳糖苷酶基因;lacA—编码β-半乳糖苷转乙酰基酶基因;lacY—编码β-半乳糖苷通透酶基因) |
| Fig. 2 Pathway diagram for the biosynthesis of LNT Ⅱ, LNnT and LNT in E. coli (ptsG—PTS glucose transporter subunit IIBC; glk—glucokinase gene; glpF—glycerol uptake facilitator protein gene; gloA—lactoylglutathione lyase gene; gloB—hydroxyacylglutathione hydrolase gene; nagE—PTS N-acetyl glucosamine transporter subunit IIABC; pfkA—6-phosphofructokinase gene; fbp—fructose-1,6-disphosphatase gene; zwf—glucose-6-phosphate dehydrogenase gene; pgi—glucose-6-phosphate isomerase gene; pgm: glucophosphomutase gene; agp—bifunctional glucose-1-phosphatase/inositol phosphatase gene; gcd—glucose dehydrogenase gene; galU—UTP-glucose-1-phosphate uridylyltransferase gene; galE—UDP-glucose 4-epimerase gene; otsA—alpha-alpha-trehalose-phosphate synthase gene; ugd—UDP-glucose 6-dehydrogenase gene; galK—galactokinase gene; galT—galactose-1-phosphate uridylyltransferase gene; glmS—L-glutamine-D-fructose-6-phosphate aminotransferase gene; glmM—phosphoglucosamine mutase gene; glmU—fused N-acetylglucosamine-1-phosphate uridyltransferase and glucosamine-1-phosphate acetyltransferase gene; nagB—glucosamine-6-phosphate deaminase gene; nagA—N-acetylglucosamine-6-phosphate deacetylase gene; murA—UDP-N-acetylglucosamine 1-carboxyvinyltransferase gene; wecB—UDP-N-acetylglucosamine 2-epimerase gene; lacZ—β-galactosidase gene; lacA—thiogalactoside transacetylase gene; lacY—lactose permease gene) |
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