Research advances in nitrogen fixation synthetic biology Synthetic Biology Journal    DOI: 10.12211/2096-8280.2025-081

Fig. 3 Constructing a symbiotic nitrogen fixation model for non-legume crops based on the nodulation symbiosis mechanism.

Other Images/Table from this Article

• Fig. 1  Main approaches to engineer or improve biological nitrogen fixation(I: Engineer nitrogen-fixing bacteria to enhance nitrogenase activity and optimize ammonium excretion efficiency; II: Strengthen crops' ability to recruit beneficial diazotrophs in the rhizosphere and regulate their nitrogen-fixing activity; III: Decipher nodulation mechanisms to achieve symbiotic nitrogen fixation in non-leguminous plants; IV: Express nitrogenase in plant organelles to enable autonomous nitrogen fixation in crops.)
• Fig. 2  The glutamine-core nitrogen metabolism regulatory pathway in diazotrophs and strategies for enhancing nitrogen fixation capacity(I: Delete or regulate the glnA gene to release free NH⁴⁺; II: Modulate the expression of NifA to enhance its activation of the nitrogenase gene cluster, thereby increasing nitrogenase activity.)