Advances in the Application of CRISPR/Cas Systems in Molecular Diagnostics Synthetic Biology Journal    DOI: 10.12211/2096-8280.2025-042

Fig. 9 The Challenges of CRISPR/Cas system in molecular diagnosis

Extracts from the Article

CRISPR/Cas系统作为近年来分子生物学领域的重要技术突破，已在分子诊断领域展现出巨大的应用潜力［130， 131］。尽管该技术在病原体检测、遗传突变识别及个体化诊疗等方面取得了诸多重要进展，但其在实际应用过程中的若干关键问题仍亟待解决，尚未完全满足临床诊断对高通量、高灵敏、便携化和低成本的综合需求。分析CRISPR/Cas系统当前存在的主要挑战［图9］，并探讨未来的可能发展方向，对于推动其在分子诊断领域的深入应用具有重要的理论与实践意义［132-134］。

Other Images/Table from this Article

• Table 1  The relation of CRISPR/Cas system effector proteins
• Fig. 1  Schematic diagram and principles of CRISPR/Cas systems for biosensing and bioimaging
• Fig. 2  Schematic of CRISPR/Cas molecular diagnostic technology based on nucleic acid pre amplification
• Fig. 3  Amplification-free CRISPR strategy without nucleic acid pre-amplification
• Fig. 4  Schematic diagram of detection methods using the CRISPR/Cas system for precise diagnosis of infectious diseases
• Fig. 5  Application of CRISPR/Cas System in Cancer Biomarker Detection
• Fig. 6  Method for detecting proteins based on CRISPR/Cas system
• Fig. 7  Antibody/metabolite detection technologies utilizing the CRISPR/Cas system
• Fig. 8  The CRISPR/Cas system combines miniaturization and integration techniques in molecular diagnostics