关键词: 自组装, 酶纳米线, “干湿”界面, 高催化活性, 有机溶剂

Abstract:

Enzymes have been regarded as key players in producing chemicals, including fine chemical products, food and pharmaceuticals, but the poor stability of three-dimensional folding structures with the enzymes impede their applications in organic systems. Maintaining enzymatic activities in organic solvents has becoming one of the urgent issues in enzyme-dependent industrial applications. Interfacial immobilization is an attractive strategy for stabilizing the structures of enzymes. Recently, an elegant strategy has been reported for constructing organic Januvia transaminase nanowires (JTAnw) with high stability in the organic system. Using the self-assembly domain of the amyloid protein Sup35 as a hydrophobic support, a unique “dry and wet” interface is constructed for JTAnws, and this interface can effectively enhance Januvia transaminase's catalytic activity, thermal stability and pH stability in different organic solvents. The work provides a smart enzyme-immobilization strategy for improving enzymes' catalysis performance in organic systems, which would expand their applications for biocatalysis in organic solvents.

Key words: self-assembly, enzymatic nanowires, “dry and wet” interfaces, high catalytic performance, organic solvents