Synthetic Biology Journal ›› 2023, Vol. 4 ›› Issue (5): 1036-1049.DOI: 10.12211/2096-8280.2023-023
• Research Article • Previous Articles
Cui MA1,2, Fan YANG1,2, Juntai ZHANG1,2, Kai HE1,2
Received:
2023-03-16
Revised:
2023-07-11
Online:
2023-11-15
Published:
2023-10-31
Contact:
Kai HE
马翠1,2, 杨凡1,2, 张君泰1,2, 何凯1,2
通讯作者:
何凯
作者简介:
基金资助:
CLC Number:
Cui MA, Fan YANG, Juntai ZHANG, Kai HE. Multifunction microplate reader for automated foundry platform[J]. Synthetic Biology Journal, 2023, 4(5): 1036-1049.
马翠, 杨凡, 张君泰, 何凯. 面向自动化铸造平台的多功能微孔板检测系统[J]. 合成生物学, 2023, 4(5): 1036-1049.
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URL: https://synbioj.cip.com.cn/EN/10.12211/2096-8280.2023-023
送板位置指令 | 回零位 |
---|---|
吸光检测孔位 | |
荧光检测孔位 | |
吸光度检测指令 | 光源(打开、关闭) |
波长选择 | |
振板(速度、时间) | |
单行检测 | |
整板检测 | |
荧光检测指令 | 光源强度设置 |
光源调试 | |
单点检测 | |
整板检测 |
Table 1 Serial port instructions
送板位置指令 | 回零位 |
---|---|
吸光检测孔位 | |
荧光检测孔位 | |
吸光度检测指令 | 光源(打开、关闭) |
波长选择 | |
振板(速度、时间) | |
单行检测 | |
整板检测 | |
荧光检测指令 | 光源强度设置 |
光源调试 | |
单点检测 | |
整板检测 |
客户端指令 | 功能介绍 |
---|---|
#Initial# | 初始化回原点 |
#Abs001# | 吸光度检测 |
#Flu001# | 荧光检测 |
#Open01# | 打开盖子 |
#Close01# | 关闭盖子 |
#Ask001# | 查询状态:工作中/结束 |
Table 2 Instructions of TCP communication
客户端指令 | 功能介绍 |
---|---|
#Initial# | 初始化回原点 |
#Abs001# | 吸光度检测 |
#Flu001# | 荧光检测 |
#Open01# | 打开盖子 |
#Close01# | 关闭盖子 |
#Ask001# | 查询状态:工作中/结束 |
波长 | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | AVE | RSD |
---|---|---|---|---|---|---|---|---|---|---|---|---|
405 nm | 0.960 | 0.964 | 0.964 | 0.966 | 0.968 | 0.965 | 0.965 | 0.965 | 0.967 | 0.963 | 0.965 | 0.0022 |
450 nm | 0.993 | 0.993 | 0.993 | 0.993 | 0.993 | 0.993 | 0.993 | 0.993 | 0.987 | 0.986 | 0.992 | 0.0027 |
492 nm | 0.997 | 0.999 | 0.997 | 0.995 | 0.997 | 0.995 | 0.997 | 0.995 | 0.994 | 0.994 | 0.994 | 0.0016 |
630 nm | 1.004 | 1.005 | 1.006 | 1.004 | 1.004 | 1.004 | 1.004 | 1.002 | 1.002 | 1.001 | 1.004 | 0.0015 |
Table 3 OD values at different wavelength
波长 | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | AVE | RSD |
---|---|---|---|---|---|---|---|---|---|---|---|---|
405 nm | 0.960 | 0.964 | 0.964 | 0.966 | 0.968 | 0.965 | 0.965 | 0.965 | 0.967 | 0.963 | 0.965 | 0.0022 |
450 nm | 0.993 | 0.993 | 0.993 | 0.993 | 0.993 | 0.993 | 0.993 | 0.993 | 0.987 | 0.986 | 0.992 | 0.0027 |
492 nm | 0.997 | 0.999 | 0.997 | 0.995 | 0.997 | 0.995 | 0.997 | 0.995 | 0.994 | 0.994 | 0.994 | 0.0016 |
630 nm | 1.004 | 1.005 | 1.006 | 1.004 | 1.004 | 1.004 | 1.004 | 1.002 | 1.002 | 1.001 | 1.004 | 0.0015 |
波长 | G2 | G4 | G6 | G8 | E2 | E4 | B2 | B4 | AVE | RSD |
---|---|---|---|---|---|---|---|---|---|---|
450 nm | 1.002 | 1.001 | 0.998 | 0.995 | 1.001 | 0.998 | 1.004 | 1.000 | 0.999 | 0.0026 |
Table 4 OD values at different position
波长 | G2 | G4 | G6 | G8 | E2 | E4 | B2 | B4 | AVE | RSD |
---|---|---|---|---|---|---|---|---|---|---|
450 nm | 1.002 | 1.001 | 0.998 | 0.995 | 1.001 | 0.998 | 1.004 | 1.000 | 0.999 | 0.0026 |
1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | |
---|---|---|---|---|---|---|---|---|
标准值 | 0.333 | 0.665 | 0.997 | 1.330 | 1.663 | 1.996 | 2.332 | 2.663 |
测量值AVE | 0.1062 | 0.2330 | 0.3332 | 0.4234 | 0.5164 | 0.5821 | 0.6543 | 0.7801 |
测量值RSD | 0.00017 | 0.00022 | 0.00030 | 0.00029 | 0.00036 | 0.00037 | 0.00072 | 0.00174 |
Table 5 OD values for different standard solutions
1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | |
---|---|---|---|---|---|---|---|---|
标准值 | 0.333 | 0.665 | 0.997 | 1.330 | 1.663 | 1.996 | 2.332 | 2.663 |
测量值AVE | 0.1062 | 0.2330 | 0.3332 | 0.4234 | 0.5164 | 0.5821 | 0.6543 | 0.7801 |
测量值RSD | 0.00017 | 0.00022 | 0.00030 | 0.00029 | 0.00036 | 0.00037 | 0.00072 | 0.00174 |
荧光素钠溶液 | 1 | 2 | 3 | 4 | 5 | AVE | RSD |
---|---|---|---|---|---|---|---|
500 pmol/L | 3.4706 | 3.4674 | 3.4709 | 3.4659 | 3.4647 | 3.4679 | 0.0028 |
250 pmol/L | 3.3776 | 3.3729 | 3.3655 | 3.3739 | 3.3666 | 3.3713 | 0.0051 |
125 pmol/L | 3.3187 | 3.3176 | 3.3108 | 3.3154 | 3.3125 | 3.3150 | 0.0033 |
62.5 pmol/L | 3.2931 | 3.2819 | 3.2828 | 3.2847 | 3.2893 | 3.2864 | 0.0047 |
31.25 pmol/L | 3.2767 | 3.2738 | 3.2782 | 3.2736 | 3.2775 | 3.2760 | 0.0021 |
15.625 pmol/L | 3.2618 | 3.2625 | 3.2688 | 3.2611 | 3.2659 | 3.2640 | 0.0032 |
7.8125 pmol/L | 3.2526 | 3.2538 | 3.2550 | 3.2544 | 3.2561 | 3.2544 | 0.0013 |
3.9063 pmol/L | 3.2516 | 3.2418 | 3.2449 | 3.2504 | 3.2522 | 3.2482 | 0.0046 |
NaOH溶液 | 3.2351 | 3.2402 | 3.2443 | 3.245 | 3.2455 | 3.2420 | 0.0044 |
Table 6 Detected values of fluorescein sodium
荧光素钠溶液 | 1 | 2 | 3 | 4 | 5 | AVE | RSD |
---|---|---|---|---|---|---|---|
500 pmol/L | 3.4706 | 3.4674 | 3.4709 | 3.4659 | 3.4647 | 3.4679 | 0.0028 |
250 pmol/L | 3.3776 | 3.3729 | 3.3655 | 3.3739 | 3.3666 | 3.3713 | 0.0051 |
125 pmol/L | 3.3187 | 3.3176 | 3.3108 | 3.3154 | 3.3125 | 3.3150 | 0.0033 |
62.5 pmol/L | 3.2931 | 3.2819 | 3.2828 | 3.2847 | 3.2893 | 3.2864 | 0.0047 |
31.25 pmol/L | 3.2767 | 3.2738 | 3.2782 | 3.2736 | 3.2775 | 3.2760 | 0.0021 |
15.625 pmol/L | 3.2618 | 3.2625 | 3.2688 | 3.2611 | 3.2659 | 3.2640 | 0.0032 |
7.8125 pmol/L | 3.2526 | 3.2538 | 3.2550 | 3.2544 | 3.2561 | 3.2544 | 0.0013 |
3.9063 pmol/L | 3.2516 | 3.2418 | 3.2449 | 3.2504 | 3.2522 | 3.2482 | 0.0046 |
NaOH溶液 | 3.2351 | 3.2402 | 3.2443 | 3.245 | 3.2455 | 3.2420 | 0.0044 |
溶液 | C11 | B19 | A9 | A1 | C3 | E5 | F6 | AVE | RSD |
---|---|---|---|---|---|---|---|---|---|
荧光素钠 | 6.5372 | 6.4458 | 6.4097 | 6.4591 | 6.5018 | 6.5274 | 6.5518 | 6.4904 | 0.0531 |
Table 7 Fluorescence values at different position
溶液 | C11 | B19 | A9 | A1 | C3 | E5 | F6 | AVE | RSD |
---|---|---|---|---|---|---|---|---|---|
荧光素钠 | 6.5372 | 6.4458 | 6.4097 | 6.4591 | 6.5018 | 6.5274 | 6.5518 | 6.4904 | 0.0531 |
检测仪 | 光吸收参数 | 荧光强度 | 自动化兼容 |
---|---|---|---|
SpectraMax Mini 多功能酶标仪[ | 检测范围(OD):0~4.0 误差<1%, OD 0~3.0 | 灵敏度: 1 pmol/L | 无相关介绍 |
BioTek Synergy H1 多功能微孔板检测仪[ | 动态范围(OD):0~4.0 误差<1%, OD 3.0 | 灵敏度:0.25 pmol/L(滤光片); 2.5 pmol/L、4 pmol/L(光栅型) | 第三方 |
山东博科公司的 BK-EL10D酶标仪[ | 读数范围:0~4.5 线性范围(OD):0~3.5 重复性:<0.2% 误差≤±0.01, OD 0.0~1.0 误差≤±0.03, OD 1.0~2.0 | 无 | 无相关介绍 |
本系统检测仪 | 检测范围(OD):0~3.0 重复性:<0.3% 误差<±0.01, OD 0~2.0 | 灵敏度:3.9 pmol/L(荧光素钠) | 接口开放,串口或 TCP实现第三方控制 |
Table 8 Performance comparison of microplate readers
检测仪 | 光吸收参数 | 荧光强度 | 自动化兼容 |
---|---|---|---|
SpectraMax Mini 多功能酶标仪[ | 检测范围(OD):0~4.0 误差<1%, OD 0~3.0 | 灵敏度: 1 pmol/L | 无相关介绍 |
BioTek Synergy H1 多功能微孔板检测仪[ | 动态范围(OD):0~4.0 误差<1%, OD 3.0 | 灵敏度:0.25 pmol/L(滤光片); 2.5 pmol/L、4 pmol/L(光栅型) | 第三方 |
山东博科公司的 BK-EL10D酶标仪[ | 读数范围:0~4.5 线性范围(OD):0~3.5 重复性:<0.2% 误差≤±0.01, OD 0.0~1.0 误差≤±0.03, OD 1.0~2.0 | 无 | 无相关介绍 |
本系统检测仪 | 检测范围(OD):0~3.0 重复性:<0.3% 误差<±0.01, OD 0~2.0 | 灵敏度:3.9 pmol/L(荧光素钠) | 接口开放,串口或 TCP实现第三方控制 |
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