Synthetic Biology Journal

   

Advance of the immobilization of hydrogenases

Hangbin LEI1, Ning HE1, Feixuan LI1, Lingling DONG1, Shizhen WANG1,2   

  1. 1.Department of Chemical and Biochemical Engineering,College of Chemistry and Chemical Engineering,Xiamen University,Xiamen,361005,Fujian,China
    2.The Key Lab for Synthetic Biotechnology of Xiamen City,Xiamen University,Xiamen,361005,Fujian,China
  • Received:2024-03-11 Revised:2024-05-17 Published:2024-06-07
  • Contact: Shizhen WANG

氢化酶固定化研究进展

雷航彬1, 何宁1, 李斐煊1, 董玲玲1, 王世珍1,2   

  1. 1.厦门大学化学化工学院化学工程与生物工程系,福建 厦门 361005
    2.厦门大学厦门市合成生物学重点实验室,福建 厦门 361005
  • 通讯作者: 王世珍
  • 作者简介:雷航彬(2000—),男,硕士研究生。研究方向为固定化酶。 E-mail:lhb20000410@qq.com
    王世珍(1982—),女,副教授,硕士生导师。研究方向为合成生物学、生物催化与转化、酶工程等。 E-mail:szwang@xmu.edu.cn
  • 基金资助:
    国家重点研发计划合成生物学重点研发项目“糖水氢电系统–体外多酶高效产氢及氢电装置的基础及工程研究”(2022YFA0912003)

Abstract:

Hydrogenases catalyze the reversible conversion of hydrogen gas into protons and electrons which is promising for industrial application. However, free hydrogenases face challenges such as oxygen sensitivity and low electron transfer rates. This review summarized the immobilization of hydrogenases by carbon materials, metals, semiconductors, polymers and metal-organic-frameworks (MOFs). Carbon materials provide the advantages of low cost and large specific surface areas, while they tend to agglomerate. Hydrogenases are immobilizated on carbon materials through adsorption, usually involving electrostatic interactions and hydrophobic interactions, and are used in bioelectrocatalysis, biofuel cells, bioreactors et al. Metals and semiconductors, known for high conductivity and excellent reactive activity, are expensive and less stable. Through adsorption involving electrostatic interaction and hydrophobic interaction, immobilization of hydrogenases on metals and semiconductors are normally applied in bioelectrocatalysis, biofuel cells, photoelectrocatalysis et al. Polymers have good biocompatibility and mechanical strength but low conductivity. Immobilization of hydrogenases on polymers can improve the stability and oxygen tolerance of hydrogenases. Immobilization on polymers is realized through adsorption and entrapment, involving hydrogen bonds, hydrophobic interactions and π-π interactions, and is often used in bioelectrocatalysis, photoelectrocatalysis et al. MOFs are designable and have high specific surface areas, which provide wide choices for hydrogenases immobilization. However, MOFs tend to collapse in harsh conditions. Immobilization on MOFs through adsorption and entrapment involves coordinate bonds, hydrophobic interaction, and π-π interaction. Furthermore, the prospect of immobilization of hydrogenases by novel hybrid materials was proposed which can expand the applications of immobilized hydrogenases. Immobilization of hydrogenases facilitates the stability of hydrogenases, which can be applied in efficient production and application of hydrogen, as well as biological asymmetric hydrogenation for chiral medicine preparation. Immobilization of hydrogenases provide alternative options for transforming energy structures, realizing green manufacturing and solving environmental problems.

Key words: immobilization of hydrogenases, bioelectrocatalysis, carbon materials, semiconductors, polymers, metal-organic frameworks

摘要:

氢化酶催化氢气向质子和电子的可逆转化,具有广阔的工业应用前景。但游离的氢化酶存在着对氧气敏感、传递电子速率慢等缺点。本文综述了碳材料、金属及半导体、高分子和金属-有机框架材料(MOFs)固定化氢化酶。碳材料具有价格低廉、比表面积大等优势。金属及半导体有着良好的导电性能和优异的催化性能。高分子材料具有良好的生物相容性和机械性能,可以提高氢化酶的稳定性和对氧气的耐受性。MOFs比表面积大,可设计调控,为理化性质不同的氢化酶提供了广泛的载体选择。复合材料固定化氢化酶可以结合不同材料的优势,拓宽固定化氢化酶的应用场景。固定化氢化酶可用于氢气的高效生产与应用以及生物不对称加氢制备手性化合物,为转变能源结构,实现绿色转型、解决环境问题提供了可选方案。

关键词: 氢化酶固定化, 生物电催化, 碳材料, 半导体材料, 高分子材料, 金属-有机框架(MOFs)

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