Synthetic Biology Journal ›› 2023, Vol. 4 ›› Issue (6): 1122-1139.DOI: 10.12211/2096-8280.2023-059

• Invited Review • Previous Articles     Next Articles

State-of-the-art for alcohol dehydrogenase development and the prospect of its applications in bio-based furan compounds valorization

Xiangshi LIU1, Yilu WU1, Peng ZHAN1, Tianhao HUANG2, Di CAI1, Peiyong QIN2   

  1. 1.National Energy R&D Center for Biorefinery,Beijing University of Chemical Technology,Beijing 100029,China
    2.Collage of Life Science and Technology,Beijing University of Chemical Technology,Beijing 100029,China
  • Received:2023-08-21 Revised:2023-10-17 Online:2024-01-19 Published:2023-12-31
  • Contact: Di CAI, Peiyong QIN

醇脱氢酶的研究进展及其催化增值生物基呋喃化合物前景展望

刘庠诗1, 吴奕禄1, 詹鹏1, 黄天灏2, 蔡的1, 秦培勇2   

  1. 1.北京化工大学,国家能源生物炼制研发中心,北京 100029
    2.北京化工大学,生命科学与技术学院,北京 100029
  • 通讯作者: 蔡的,秦培勇
  • 作者简介:刘庠诗(2000—),女,硕士研究生。研究方向为光/电-生物催化生物基平台化合物合成。E-mail:lxs20000417@163.com
    蔡的(1989—),男,副教授。研究方向主要为绿色生物制造。E-mail:caidibuct@163.com
    秦培勇(1976—),男,教授。研究方向主要为生物化工和膜分离等。E-mail:qinpy@mail.buct.edu.cn
  • 基金资助:
    国家自然科学基金(22078018);北京自然科学基金(2222016)

Abstract:

Alcohol dehydrogenase (ADH) is found widely in living cells, where it catalyzes the oxidation or reduction between hydroxyl group and carbonyl group. It also catalyzes redox reactions of a variety of organic compounds with high selectivity. In recent years, with the research progress of the catalytic mechanism, structural information, molecular modification, and reaction systems, ADH has shown great promise in the highly selective catalysis of various bio-based platform compounds. One example is the oxidation or reduction of furan derivatives such as furfural and 5-hydroxymethylfurfural, which are important sustainable building blocks for bio-jet fuels and biomaterials that are derived from tandem hydrolysis, isomerization, and dehydration of hemicelluloses and celluloses fractions in lignocellulose matrixes. This review focuses on the cutting-edge technologies in molecular design and directional engineering of ADH. In addition, the intensification of cofactor regeneration processes, including chemical-driven, enzyme-driven, and photo/electricity-driven pathways were also summarized. These methods could be the solutions to the negative aspects, such as expensive cost, poor stability, and the poor circulating efficiency of the nicotinamide cofactors that are indispensable assisted in typical ADH catalysis process. Moreover, the latest research progresses of ADH in catalysis of bio-based furans platform chemicals were also discussed. Apart from using ADH solely for the activation of the hydroxyl and carbonyl groups in the biobased furan derivates and the production of oxidative and reductive products, there is also of great promise in cascade ADH catalysis and other chemical or biological catalysis processes in one-pot under relatively mild conditions to valorize the furan derivates into valuable fine chemicals. Meanwhile, the whole-cell catalytic process that involves ADH and in vivo cofactors regeneration also possesses potentials in biobased furans valorization, with the advantages of low catalyst loading and processing costs. Overall, the researches of ADH in catalysis biological furans valorization has entered a new stage. With the further exploration of the potential applications of ADH, its role in the transformation of biomass resources will be increasingly important, particularly in the industrial process in the future.

Key words: alcohol dehydrogenase, furan compounds, oxidation-reduction reaction, cofactor regeneration, biomass valorization

摘要:

醇脱氢酶(alcohol dehydrogenase, ADH)广泛存在于生物体内,可应用于多种有机物选择性氧化还原。近年来,随着对酶的催化机理、结构认知、分子改造和反应系统构建及强化等方面研究的逐渐深入,ADH在生物基平台化合物的高选择性催化氧化还原方面展现出巨大潜力。本文综述了ADH分子设计和定向改造的前沿技术和进展,面向常见的辅因子依赖性ADH催化过程中的辅因子高成本及稳定性差等局限,聚焦酶反应过程中的辅因子再生强化技术,梳理了适用于ADH催化系统的化学驱动、酶驱动和光电驱动辅因子再生路径,并从单酶催化体系开发、多酶协同催化系统挖掘、全细胞催化技术发展与应用等多个角度概述了ADH在催化生物基呋喃化合物方向的最新研究进展。随着对ADH应用潜力的进一步挖掘,未来ADH有望成为生物基呋喃增值工业化进程中的重要组成部分,为能源生产的绿色发展提供助力。

关键词: 醇脱氢酶, 呋喃类化合物, 氧化还原反应, 辅因子再生, 生物质增值

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