1 |
YAMANAKA K, REYNOLDS K A, KERSTEN R D, et al. Direct cloning and refactoring of a silent lipopeptide biosynthetic gene cluster yields the antibiotic taromycin A[J]. Proceedings of the National Academy of Sciences of the United States of America, 2014, 111(5): 1957-1962.
|
2 |
LEE N C O, LARIONOV V, KOUPRINA N. Highly efficient CRISPR/Cas9-mediated TAR cloning of genes and chromosomal loci from complex genomes in yeast[J]. Nucleic Acids Research, 2015, 43(8): e55.
|
3 |
FU Jun, BIAN Xiaoying, HU Shenbiao, et al. Full-length RecE enhances linear-linear homologous recombination and facilitates direct cloning for bioprospecting[J]. Nature Biotechnology, 2012, 30(5): 440-446.
|
4 |
JIANG Wenjun, ZHAO Xuejin, GABRIELI T, et al. Cas9-Assisted Targeting of CHromosome segments CATCH enables one-step targeted cloning of large gene clusters[J]. Nature Communications, 2015, 6: 8101.
|
5 |
JIANG Wenjun, ZHU Ting F. Targeted isolation and cloning of 100-kb microbial genomic sequences by Cas9-assisted targeting of chromosome segments[J]. Nature Protocols, 2016, 11(5): 960-975.
|
6 |
WANG Hailong, LI Zhen, JIA Ruonan, et al. ExoCET: exonuclease in vitro assembly combined with RecET recombination for highly efficient direct DNA cloning from complex genomes[J]. Nucleic Acids Research, 2018, 46(5): e28.
|
7 |
TAO Weixin, CHEN Li, ZHAO Chunhua, et al. In vitro packaging mediated one-step targeted cloning of natural product pathway[J]. ACS Synthetic Biology, 2019, 8(9): 1991-1997.
|
8 |
ENGHIAD B, HUANG Chunshuai, GUO Fang, et al. Cas12a-assisted precise targeted cloning using in vivo Cre-lox recombination[J]. Nature Communications, 2021, 12(1): 1171.
|
9 |
GREUNKE C, DUELL E R, D'AGOSTINO P M, et al. Direct Pathway Cloning (DiPaC) to unlock natural product biosynthetic potential[J]. Metabolic Engineering, 2018, 47: 334-345.
|
10 |
D'AGOSTINO P M, GULDER T A M. Direct pathway cloning combined with sequence- and ligation-independent cloning for fast biosynthetic gene cluster refactoring and heterologous expression[J]. ACS Synthetic Biology, 2018, 7(7): 1702-1708.
|
11 |
QUAN Jiayuan, TIAN Jingdong. Circular polymerase extension cloning for high-throughput cloning of complex and combinatorial DNA libraries[J]. Nature Protocols, 2011, 6(2): 242-251.
|
12 |
SHAO Zengyi, ZHAO Hua, Zhao Huimin. DNA assembler, an in vivo genetic method for rapid construction of biochemical pathways[J]. Nucleic Acids Research, 2009, 37(2): e16.
|
13 |
SHAO Zengyi, ZHAO Huimin. Manipulating natural product biosynthetic pathways via DNA assembler[J]. Current Protocols in Chemical Biology, 2014, 6(2): 65-100.
|
14 |
HIPPEL P H VON, JOHNSON N P, MARCUS A H. Fifty years of DNA “breathing”: reflections on old and new approaches[J]. Biopolymers, 2013, 99(12): 923-954.
|
15 |
GODISKA R, MEAD D, DHODDA V, et al. Linear plasmid vector for cloning of repetitive or unstable sequences in Escherichia coli [J]. Nucleic Acids Research, 2009, 38(6): e88.
|
16 |
HAO Tingting, XIE Zhoujie, WANG Min, et al. An anaerobic bacterium host system for heterologous expression of natural product biosynthetic gene clusters[J]. Nature Communications, 2019, 10: 3665.
|
17 |
ZHANG Youming, BUCHHOLZ F, MUYRERS J P P, et al. A new logic for DNA engineering using recombination in Escherichia coli [J]. Nature Genetics, 1998, 20(2): 123-128.
|
18 |
ZHANG Youming, MUYRERS J P, TESTA G, et al. DNA cloning by homologous recombination in Escherichia coli [J]. Nature Biotechnology, 2000, 18(12): 1314-1317.
|
19 |
WANG Hailong, LI Zhen, JIA Ruonan, et al. RecET direct cloning and Redαβ recombineering of biosynthetic gene clusters, large operons or single genes for heterologous expression[J]. Nature Protocols, 2016, 11(7): 1175-1190.
|
20 |
CHAI Yi, SHAN Shiping, WEISSMAN K J, et al. Heterologous expression and genetic engineering of the tubulysin biosynthetic gene cluster using red/ET recombineering and inactivation mutagenesis[J]. Chemistry & Biology, 2012, 19(3): 361-371.
|
21 |
SONG Chaoyi, LUAN Ji, CUI Qingwen, et al. Enhanced heterologous spinosad production from a 79-kb synthetic multi-operon assembly[J]. ACS Synthetic Biology, 2019, 8(1): 137-147.
|
22 |
FU Jun, WENZEL S C, PERLOVA O, et al. Efficient transfer of two large secondary metabolite pathway gene clusters into heterologous hosts by transposition[J]. Nucleic Acids Research, 2008, 36(17): e113.
|
23 |
ABBASI M N, FU Jun, BIAN Xiaoying, et al. Recombineering for genetic engineering of natural product biosynthetic pathways[J]. Trends in Biotechnology, 2020, 38(7): 715-728.
|
24 |
SONG Chaoyi, LUAN Ji, LI Ruijuan, et al. RedEx: a method for seamless DNA insertion and deletion in large multimodular polyketide synthase gene clusters[J]. Nucleic Acids Research, 2020, 48(22): e130.
|
25 |
WANG Hailong, BIAN Xiaoying, XIA Liqiu, et al. Improved seamless mutagenesis by recombineering using ccdB for counterselection[J]. Nucleic Acids Research, 2013, 42(5): e37.
|
26 |
BIAN Xiaoying, PLAZA A, YAN Fu, et al. Rational and efficient site-directed mutagenesis of adenylation domain alters relative yields of luminmide derivatives in vivo[J]. Biotechnology and Bioengineering, 2015, 112(7): 1343-1353.
|
27 |
NEWMAN D J, CRAGG G M. Natural products as sources of new drugs over the nearly four decades from 01/1981 to 09/2019[J]. Journal of Natural Products, 2020, 83(3): 770-803.
|
28 |
HUO Liujie, HUG J J, FU Chengzhang, et al. Heterologous expression of bacterial natural product biosynthetic pathways[J]. Natural Product Reports, 2019, 36(10): 1412-1436.
|
29 |
LI J S, DU Yongle, GU Di, et al. Discovery and biosynthesis of clostyrylpyrones from the obligate anaerobe clostridium roseum[J]. Organic Letters, 2020, 22(21): 8204-8209.
|
30 |
ZHANG Weimin, ZHAO Guanghou, LUO Zhouqing, et al. Engineering the ribosomal DNA in a megabase synthetic chromosome[J]. Science, 2017, 355(6329): eaaf3981.
|
31 |
XIE Zexiong, LI Bingzhi, MITCHELL L A, et al. “Perfect” designer chromosome V and behavior of a ring derivative[J]. Science, 2017, 355(6329): eaaf4704.
|
32 |
WU Yi, LI Bingzhi, ZHAO Meng, et al. Bug mapping and fitness testing of chemically synthesized chromosome X[J]. Science, 2017, 355(6329): eaaf4706.
|
33 |
SHEN Yue, WANG Yun, CHEN Tai, et al. Deep functional analysis of synII, a 770-kilobase synthetic yeast chromosome[J]. Science, 2017, 355(6329): eaaf4791.
|
34 |
GIBSON D G, GLASS J I, LARTIGUE C, et al. Creation of a bacterial cell controlled by a chemically synthesized genome[J]. Science, 2010, 329(5987): 52-56.
|
35 |
FREDENS J, WANG Kaihang, DE LA TORRE D, et al. Total synthesis of Escherichia coli with a recoded genome[J]. Nature, 2019, 569(7757): 514-518.
|