Abstract:

Enzymes have been regarded as key players in producing chemicals, including fine chemical products, food and pharmaceuticals, but the poor stability of three-dimensional folding structures with the enzymes impede their applications in organic systems. Maintaining enzymatic activities in organic solvents has becoming one of the urgent issues in enzyme-dependent industrial applications. Interfacial immobilization is an attractive strategy for stabilizing the structures of enzymes. Recently, an elegant strategy has been reported for constructing organic Januvia transaminase nanowires (JTAnw) with high stability in the organic system. Using the self-assembly domain of the amyloid protein Sup35 as a hydrophobic support, a unique “dry and wet” interface is constructed for JTAnws, and this interface can effectively enhance Januvia transaminase's catalytic activity, thermal stability and pH stability in different organic solvents. The work provides a smart enzyme-immobilization strategy for improving enzymes' catalysis performance in organic systems, which would expand their applications for biocatalysis in organic solvents.

Key words: self-assembly, enzymatic nanowires, “dry and wet” interfaces, high catalytic performance, organic solvents

摘要：

酶作为一类高效的生物催化剂，是化学工业生产中的主要驱动力之一，被广泛用于精细化工、食品加工和医药生产等多个领域。然而，酶的三维活性结构极易被有机溶剂破坏，严重阻碍了其在含有机溶剂的工业生产中的应用。如何保持有机溶剂催化反应体系中的酶活性，是有机相工业生产中亟待解决的问题。酶的固定化被认为是保留酶稳定性的有效手段之一。近期，张先恩和门冬团队利用具有自组装特性的酵母朊蛋白Sup35为基质，构建了一种兼具亲疏水复合界面结构的Januvia转氨酶（JTA）纳米线。JTA纳米线在多种有机溶剂体系中呈现出优异的催化活性，且具有良好的热稳定性和pH稳定性。该工作为有机体系中的酶催化提供了一种优异的酶固定策略，拓宽了酶催化在有机相工业生产中的应用前景。

关键词: 自组装, 酶纳米线, “干湿”界面, 高催化活性, 有机溶剂