Biosynthesis of xylo-oligosaccharides from wheat straw xylan via the synergistic hydrolysis by xylanase Xyn11A and arabinofuranosidase Abf62A Synthetic Biology Journal    DOI: 10.12211/2096-8280.2025-037

Fig. 2 Abf62A enzyme activity of different strains

Extracts from the Article

Using the wheat xylan solution of 10 g/L as the substrate， the protease activity was detected. The results were showcased in Fig. 2. It was obvious that three strains had higher protease activity. The highest protease activity was 10 U/mL， and the lowest protease activity was 5 U/mL. Consequently， strain 4 was selected for subsequent culture optimization.

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• Fig. 1  Cloning and expression analysis of Abf62A in P. pastoris
• Fig. 3  Effects of culture time (a) and temperature (b) on the enzyme activity of Abf62A fermentation liquor.
• Fig. 4  SDS-PAGE results Abf62A protein(Lane A: Abf62A fermentation liquor; Lane B: Abf62A flow-through solution; Lane C: Abf62A eluate solution; Lane D: Abf62A pure enzyme solution)
• Fig. 5  The optimum pH and pH stability of Abf62A (a) and its optimum temperature and temperature stability (b)
• Fig. 6  Integrated process for valorizing wheat straw into antioxidant xylooligosaccharides
• Table 1  Analysis of Xyn11A enzymatic hydrolysis products of different WS xylan
• Table 2  Sugar content and proportion in xylan hydrolysates of different WS.
• Fig. 7  Radical scavenging activity of xylo-oligosaccharides (XOS)(Data represent mean values of triplicate measurements.)