Biosynthesis of xylo-oligosaccharides from wheat straw xylan via the synergistic hydrolysis by xylanase Xyn11A and arabinofuranosidase Abf62A Synthetic Biology Journal    DOI: 10.12211/2096-8280.2025-037

Fig. 4 SDS-PAGE results Abf62A protein
(Lane A: Abf62A fermentation liquor; Lane B: Abf62A flow-through solution; Lane C: Abf62A eluate solution; Lane D: Abf62A pure enzyme solution)

Extracts from the Article

The specific enzyme activity of Abf62A fermentation liquor expressed under optimization of culture conditions was 14.9 U/mL （or 159.0 U/mg protein）， which was 43% higher than that of control （10 U/mL）. It could be seen from Fig. 4 lane A that there were some number of miscellaneous proteins in the crude enzyme solution of Abf62A， but there was also Abf62A in the flow-through fluid （Fig. 4 lane B）， indicating that the target proteins Abf62A didn’t bind to the chromatographic column well during the chromatography process. For all this， the Abf62A eluate solution was purified to electrophoretic purity （Fig. 4 lane C）. After ultrafiltration concentration of Abf62A eluate solution， the specific enzyme activity of Abf62A pure enzyme solution （Fig. 4 lane D） was 347.9 U/mL （or 173.5 U/mg protein）.

Other Images/Table from this Article

• Fig. 1  Cloning and expression analysis of Abf62A in P. pastoris
• Fig. 2  Abf62A enzyme activity of different strains
• Fig. 3  Effects of culture time (a) and temperature (b) on the enzyme activity of Abf62A fermentation liquor.
• Fig. 5  The optimum pH and pH stability of Abf62A (a) and its optimum temperature and temperature stability (b)
• Fig. 6  Integrated process for valorizing wheat straw into antioxidant xylooligosaccharides
• Table 1  Analysis of Xyn11A enzymatic hydrolysis products of different WS xylan
• Table 2  Sugar content and proportion in xylan hydrolysates of different WS.
• Fig. 7  Radical scavenging activity of xylo-oligosaccharides (XOS)(Data represent mean values of triplicate measurements.)