Advances in electro-microbial synergistic systems for value-added conversion of carbon dioxide Synthetic Biology Journal    DOI: 10.12211/2096-8280.2025-070

Fig. 10 An integrated electromicrobial process for converting CO₂ into higher alcohols^[90]

Extracts from the Article

基于上述优势，研究者已构建出多种高效集成化反应器，用于CO₂向高附加值化合物的高效转化。Li 等人率先设计并实现了一种典型的原位一体化反应器集成系统，其中将电催化模块与微生物催化模块集成于一个密闭反应器内（图10）［90］。在该系统中，CO₂首先在电催化阴极上还原为甲酸，作为中间碳源供给后续的生物合成；同时，选用具有固碳与能量积累能力的R. eutropha H16作为工程宿主，通过代谢工程破坏其PHB合成路径，并增强其对甲酸的摄取与代谢能力，使其更专一地将甲酸转化为目标燃料。具体而言，经过定向改造的R. eutropha H16菌株在以CO₂和电能为唯一碳源与能源的条件下，实现了异丁醇与3-甲基-1-丁醇等高值生物燃料的合成，终产物浓度超过140 mg/L，验证了该系统在碳中和与绿色能源制备方面的潜力。

Other Images/Table from this Article

• Fig.1  Classifications of electrocatalytic-microbial in situ coupling systems
• Fig.2  Design of different electrode materials and schematic diagram of the MES systems
• Fig.3  Design of artificially regulated MES systems
• Fig. 4  MES for C₂₊ product synthesis
• Fig. 5  H₂-mediated enhancement strategies for MES-1
• Fig. 6  H₂-mediated enhancement strategies for MES-2
• Fig. 7  Schematic of enhanced CO₂-to-CH₄ energy efficiency via redox-mediated cathode functionalization in MES^[80]
• Fig. 8  Construction of an artificial photosynthesis system by integrating a photoelectrochemical system with genetically engineered cells expressing rhodopsin and an outer-membrane conduit MtrCAB^[95]
• Fig. 9  Three integrated modes of electrocatalytic-microbial ex situ coupling systems for CO₂ conversion^[9]
• Fig. 11  Schematic illustration of the integrated EMC2 system^[96]
• Fig. 12  Schematic of the continuous-flow biohybrid CO₂ electrolysis-fermentation system^[102]
• Fig. 13  Schematic illustration of the electromicrobial cascade system for artificial glucose synthesis^[103]
• Fig. 14  Schematic of the spatially separated electrochemical CO₂ reduction reaction (CO₂RR) and microbial fermentation process for efficient β-farnesene synthesis from CO₂^[98]
• Fig. 15  Schematic of the sequential CO₂ electrolysis and microbial fermentation system for artificial synthesis of PHB^[104]
• Fig. 16  Schematic illustration of L-tyrosine synthesis from CO₂ using a blended nexus molecular system based on an abiotic/biotic cascade catalysis^[105]
• Fig. 17  Schematic diagram of the carbon dioxide electrocatalytic platform and microbial conversion for long-chain compound synthesis. a) Schematic illustration of the integrated electrocatalytic/biocatalytic platform system for the synthesis of long-chain compounds from CO₂. b) Schematic depiction of the fabrication process of the MPN@deCOP@Ag-Cu₂O electrocatalytic platform. c) Construction of the ethanol utilization pathway^[106].
• Table 1  Representative work: in situ vs ex situ coupling
• Table 2  Comparative analysis of in situ and ex situ coupling systems