生物制造的PE值与PX值：定义与应用

doi:10.12211/2096-8280.2025-020

合成生物学 ›› 2025, Vol. 6 ›› Issue (4): 715-727.DOI: 10.12211/2096-8280.2025-020

生物制造的PE值与PX值：定义与应用

张以恒¹^,²(), 陈雪梅², 韩平平¹^,²

^1.中国科学院天津工业生物技术研究所，低碳合成工程生物学全国重点实验室，天津 300308
^2.中国科学院天津工业生物技术研究所，体外合成生物学中心，天津 300308

收稿日期:2025-03-20 修回日期:2025-04-29 出版日期:2025-08-31 发布日期:2025-09-03
通讯作者: 张以恒
作者简介:张以恒（1971—），低碳合成工程生物学全国重点实验室主任，中国科学院天津工业生物技术研究所体外合成生物学中心主任，曾经任美国弗吉尼亚理工大学终身正教授。他是体外合成生物学的奠基人之一与产业化领跑者，创建体外生物转化（ivBT）技术平台，率先提出“人工多酶分子机器”概念并实现产业化突破。在秸秆制粮、体外无细胞呼吸作用（无氧呼吸作用产氢、有氧呼吸作用制电）、人工合成淀粉、淀粉制塔格糖与肌醇等方向取得了一系列原创性突破。E-mail：zhang_xw@tib.cas.cn
基金资助:
国家重点研发计划“合成生物学”重点专项(2022YFA0912300);国家自然科学基金面上项目(NSFC32271544);合成生物学海河实验室颠覆性创新项目(22HHSWSS000155);天津市合成生物技术创新能力提升行动项目(TSBICIP-CXRC-067)

PE and PX values in biomanufacturing: definitions and applications

ZHANG Yi-Heng P. Job¹^,²(), CHEN Xuemei², HAN Pingping¹^,²

^1.State Key Laboratory of Engineering Biology for Low-Carbon Manufacturing，Tianjin Institute of Industrial Biotechnology，Chinese Academy of Sciences，Tianjin 300308，China
^2.In vitro Synthetic Biology Center，Tianjin Institute of Industrial Biotechnology，Chinese Academy of Sciences，Tianjin 300308，China

Received:2025-03-20 Revised:2025-04-29 Online:2025-08-31 Published:2025-09-03
Contact: ZHANG Yi-Heng P. Job

摘要/Abstract

摘要：

生物制造是战略新兴产业的典型代表，是生物经济的新质生产力。作者曾提出“道法术器”对工业生物制造的哲学指导意义。为进一步阐述生物制造中“术以立策”的原则，本文首次提出衡量生物催化剂（即“术”）水平的关键经济指标——PE值（product-to-enzyme ratio）与PX值［product-to-X（cell）ratio］。这两个指标具有简单、透明且量化的属性。PE值表示产品与非细胞催化剂（酶分子或多酶分子机器，以下简称“多酶机器”）的质量比值，也可通过技术指标总转换数（total turn-over number，TTN）计算其理论值。PX值应用于细胞工厂发酵，表示产品与细胞催化剂的质量比。基于PE值与PX值，可以快速估算不同生物制造过程中的生物催化剂成本，进而指导降低降本增效的关键路径。作者汇总了生物制造的产业案例及文献数据，展示了酶分子及多酶分子机器PE值、细胞工厂PX值的特点。研究表明，淀粉酶水解淀粉的PE值是纤维素酶水解纤维素的50～100倍；在固态纤维素水解过程中，纤维素酶的超大用量是非粮生物质糖化与利用的最大经济障碍。最后，本文探讨了PE值的技术改进路径，特别是多酶共固定技术的潜力，并明确了工业酶皇冠——纤维素酶研究的新方向。PE值与PX值的分析将为生物制造战略新兴产业的发展提供全新的视角，深化了对生物制造中关键“芯片”——生物催化剂成本的理解，为未来技术的发展提供重要参考与指导。

关键词: 生物制造, PE值, PX值, 道法术器, 多酶分子机器

Abstract:

Biomanufacturing is one of the representatives of strategic emerging industries and represents a new quality productive force for the upcoming bioeconomy. The authors used to propose the philosophical guiding significance of “Tao-Fa-Shu-Qi” for industrial biomanufacturing. To further elaborate on the principle of “Shu is techniques” in biomanufacturing, for the first time this opinion paper introduces the new concepts of “Product-to-Enzyme Ratio” (abbreviated as PE value) that is the weight ratio of the product to the non-cell biocatalysts (i.e., enzyme(s)), such as enzyme molecules and multiple-enzyme molecular machines, and of “Product-to-X(Cell) Ratio” (abbreviated as PX value) that is the weight ratio of the product to cell mass. Both values feature simple, clear and quantitative properties. The PE value can be applied to enzyme-based biocatalysis or in vitro biotransformation; PX value can be applied to cell-based fermentation. Theoretical PE values can be calculated from total-turn-over number (TTN) of enzyme. First, the author defines and calculates the PE value. Second, authors summarize and classify industrial biomanufacturing cases and literature data catalyzed by enzymes, multiple-enzyme molecular machines, and cells. It was found that enzymatic starch-hydrolyzing enzymes has a PE value 50-100 times of that of enzymatic cellulose hydrolysis, resulting in an extremely high cellulase dosage. This ultra-high enzyme cost is the biggest obstacle to the industrialization of the second-generation biorefineries that are based on the biological conversion and utilization of lignocellulosic biomass. Lastly, the PE/PX values can be used to quickly estimate and compare the costs of biocatalysts, such as enzyme molecules, multiple-enzyme molecular machines, and microbial cells in the biomanufacturing process, guiding the development path for further decreasing the cost of biocatalysts. Increasing PE values by enzyme (co-)immobilization could greatly decrease biocatalyst costs, surpassing cell-based fermentation. Cellulase could be the largest industrial enzyme with a potential market size of 500 billion RMB by considering the huge supplies of non-food lignocellulosic biomass and potential markets of renewable biocommodities and artificial food/feed. The calculation and analysis of the PE/PX values would provide a new perspective for the future development of the strategic emerging industry of biomanufacturing, deepen the understanding of the cost of biocatalysts in biomanufacturing, promote the high-quality development of the bioeconomy, predict the future cost trend of biological products, and evaluate the industrialization potential of emerging biotechnologies. {L-End}

Key words: biomanufacturing, PE value, PX value, Tao-Fa-Shu-Qi, multiple-enzyme molecular machine

中图分类号:

张以恒, 陈雪梅, 韩平平. 生物制造的PE值与PX值：定义与应用[J]. 合成生物学, 2025, 6(4): 715-727.

ZHANG Yi-Heng P. Job, CHEN Xuemei, HAN Pingping. PE and PX values in biomanufacturing: definitions and applications[J]. Synthetic Biology Journal, 2025, 6(4): 715-727.

图/表 5

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比较内容	分泌酶	胞内酶
代表性菌	毕赤酵母、里氏木霉、枯草芽孢杆菌、黑曲霉	大肠杆菌、枯草芽孢杆菌
代表性酶制剂	植酸酶、纤维素酶、木聚糖酶、蛋白酶、液化酶、糖化酶、脂肪酶、果胶酶、漆酶等	木糖异构酶、核酸酶、SOD、甲酸脱氢酶、醇脱氢酶等
蛋白表达水平	200 g/L（最高），一般在10～50 g/L左右	5～10 g/L（最高），大多数在1～5 g/L左右
蛋白制造成本^[1,7-8]	约100元/kg酶干重	约250～1000元/kg酶干重
酶分离难易程度	容易，低成本	比较贵
目标酶	有限制，与底盘细胞的分泌同源酶序列高度相似	普适性
技术难点	基因操作难，要长期（几年以上）系统优化	基因操作相当容易
蛋白表达的比喻	牛产奶	杀牛产肉

比较内容	分泌酶	胞内酶
代表性菌	毕赤酵母、里氏木霉、枯草芽孢杆菌、黑曲霉	大肠杆菌、枯草芽孢杆菌
代表性酶制剂	植酸酶、纤维素酶、木聚糖酶、蛋白酶、液化酶、糖化酶、脂肪酶、果胶酶、漆酶等	木糖异构酶、核酸酶、SOD、甲酸脱氢酶、醇脱氢酶等
蛋白表达水平	200 g/L（最高），一般在10～50 g/L左右	5～10 g/L（最高），大多数在1～5 g/L左右
蛋白制造成本^[1,7-8]	约100元/kg酶干重	约250～1000元/kg酶干重
酶分离难易程度	容易，低成本	比较贵
目标酶	有限制，与底盘细胞的分泌同源酶序列高度相似	普适性
技术难点	基因操作难，要长期（几年以上）系统优化	基因操作相当容易
蛋白表达的比喻	牛产奶	杀牛产肉

产物	细胞工厂	PX值
重组蛋白	大肠杆菌	0.1～0.2
全蛋白（可食用）	SCP生产菌	0.4～0.65
α-淀粉酶	枯草芽孢杆菌	0.5～2
糖化酶	黑曲霉	0.5～2
植酸酶	毕赤酵母	1
青霉素	产黄青霉	1～2
蛋白酶	枯草芽孢杆菌	1～3
丁二酸	大肠杆菌	2～3
纤维素酶	里氏木霉	2～4
赖氨酸	大肠杆菌	2～4
谷氨酸	谷氨酸棒杆菌	3～4
乙醇	酿酒酵母	5～10
乳酸	大肠杆菌	5～10
柠檬酸	黑曲霉	8～10
L-丙氨酸	大肠杆菌	约10

产物	细胞工厂	PX值
重组蛋白	大肠杆菌	0.1～0.2
全蛋白（可食用）	SCP生产菌	0.4～0.65
α-淀粉酶	枯草芽孢杆菌	0.5～2
糖化酶	黑曲霉	0.5～2
植酸酶	毕赤酵母	1
青霉素	产黄青霉	1～2
蛋白酶	枯草芽孢杆菌	1～3
丁二酸	大肠杆菌	2～3
纤维素酶	里氏木霉	2～4
赖氨酸	大肠杆菌	2～4
谷氨酸	谷氨酸棒杆菌	3～4
乙醇	酿酒酵母	5～10
乳酸	大肠杆菌	5～10
柠檬酸	黑曲霉	8～10
L-丙氨酸	大肠杆菌	约10

酶类别	酶名	底物	产物	酶形式	酶数量	k_cat/s^-1	k_d/s^-1	TTN	MW_P/Da	MW_E/kDa	PE值^① （理论）	PE值^② （实际）	参考文献
异构酶	葡萄糖磷酸异构酶	果糖-6-磷酸	葡萄糖-6-磷酸	游离	1	2765	8.0×10^-5	3.4E×10⁷	260.14	48	1.9×10⁵	NA	[12]
	葡萄糖磷酸异构酶	果糖-6-磷酸	葡萄糖-6-磷酸	固定化	1	2198	1.0×10^-6	2.2×10⁹	260.14	67	8.4×10⁶	NA	[12]
	D-塔格糖-3-差向异构酶	D-果糖	D-阿洛酮糖	游离	1	55.1	1.7×10^-4	3.3×10⁵	180	33	1.8×10³	NA	[13]
	D-塔格糖-3-差向异构酶	D-果糖	D-阿洛酮糖	固定化	1	93.4	3.9×10^-5	2.4×10⁶	180	33	1.3×10⁴	1000	[13]
	木糖异构酶	D-葡萄糖	D-果糖	游离	1	30	7.7×10^-6	3.9×10⁶	180	46	1.5×10⁴	NA	[14]
	木糖异构酶	D-葡萄糖	D-果糖	固定化	1	27	1.0×10^-6	2.7×10⁷	180	46	1.1×10⁵	1.0×10⁵	[15]
水解酶	青霉素G酰胺酶	青霉素G	6-氨基青霉烷酸	游离	1	44.6	1.1×10^-4	4.0×10⁵	216.25	88	9.8×10²	NA	[16-17]
	青霉素G酰胺酶	青霉素G	6-氨基青霉烷酸	固定化	1	NA	NA	NA	NA	NA	NA	600	[16,18]
	脂肪酶	油脂+醇类	脂肪酸酯	游离	1	500	4.1×10^-5	1.2×10⁷	250	43	6.9×10⁴	150	[16,19]
	脂肪酶	油脂+醇类	脂肪酸酯	固定化	1	NA	NA	NA	NA	NA	NA	1000～5000	[16]
异构酶	核糖磷酸异构酶	核酮糖-5-磷酸	核糖-5-磷酸	游离	1	540	2.7×10^-6	2.0×10⁸	230.1	15.9	2.9×10⁶	NA	[20]
异构酶	葡萄糖磷酸变位酶	葡萄糖-1-磷酸	葡萄糖-6-磷酸	游离	1	190	2.7×10^-6	7.1×10⁷	260.14	64.9	2.8×10⁵	NA	[21]
水解酶	果糖-1,6-二磷酸酶	果糖-1,6-二磷酸	果糖-6-磷酸	游离	1	8.57	1.4×10^-7	5.9×10⁷	260.14	28	5.5×10⁵	NA	[22]
水解酶	β-糖苷酶	纤维二糖	葡萄糖	游离	1	208	3.5×10^-5	6.0×10⁶	180	62.78	1.7×10⁴	NA	[23]
氧化还原酶	肉桂醇脱氢酶	肉桂醇	肉桂醛	游离	1	18	2.00×10^-3	9.0×10³	132.159	80	15	NA	[24]
	氢酶	H⁺	氢气	游离	1	1030	2.7×10^-6	3.9×10⁸	2	110	7.0×10³	NA	[25]
	胺脱氢酶	NAD⁺	NADH	游离	1	167	1.1×10^-6	1.5×10⁸	663.43	45	2.1×10⁶	NA	[26]
	6-磷酸葡萄糖酸脱氢酶	葡萄糖6-磷酸	6-磷酸葡萄糖酸	游离	1	325	1.4×10^-6	2.4×10⁸	495.3	53	2.2×10⁶	NA	[27]
转移酶	转醛缩酶	果糖-6-磷酸，赤藓糖-4-磷酸	景天糖-7-磷酸	游离	1	22.3	1.5×10^-5	1.5×10⁶	290.16	24	1.8×10⁴	NA	[28]
混合酶	伊沙替韦酶	2-乙酰甘油	伊沙替韦	游离	9	NA	NA	NA	NA	NA	NA	1	[29]
	PHB合成	淀粉	PHB	游离	19～20	NA	NA	NA	NA	NA	NA	1	[30]
	合成淀粉多酶机器	CO₂+H₂	合成淀粉	游离	13	NA	NA	NA	NA	NA	NA	1	[31]
	单萜烯酶	葡萄糖	单萜烯	游离	27	NA	NA	NA	NA	NA	NA	2	[32]
	肌醇多酶机器	淀粉	肌醇	游离	4～6	1.1	2.0×10^-5	5.5×10⁴	180	50	196	13	[33]
	肌醇多酶机器	淀粉	肌醇	固定化	4～6	1.2	3.5×10^-6	3.4×10⁵	180	50	1242	60～80	[34]
	D-塔格糖多酶机器	淀粉	D-塔格糖	游离	5～6	NA	NA	NA	NA	NA	NA	35	[35]
	D-塔格糖多酶机器	淀粉	D-塔格糖	固定化	4～6	NA	NA	NA	NA	NA	NA	103	[35]
	纤维素酶（混合）	（预处理）生物质	D-葡萄糖	游离	3～10	NA	NA	NA	NA	NA	NA	50～100	[36-37]
	淀粉酶（混合）	（糊化）淀粉	D-葡萄糖	游离	2～3	NA	NA	NA	NA	NA	NA	5000	[18]

生物制造的PE值与PX值：定义与应用

PE and PX values in biomanufacturing: definitions and applications

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