Synthetic Biology Journal ›› 2022, Vol. 3 ›› Issue (4): 728-747.DOI: 10.12211/2096-8280.2021-094
• Invited Review • Previous Articles Next Articles
Zhaoying YANG1,2, Fan ZHANG1,2, Jianwen GUO1,2, Weiping GAO1
Received:
2021-09-27
Revised:
2021-11-11
Online:
2022-09-08
Published:
2022-08-31
Contact:
Weiping GAO
杨兆颖1,2, 张帆1,2, 郭建文1,2, 高卫平1
通讯作者:
高卫平
作者简介:
基金资助:
CLC Number:
Zhaoying YANG, Fan ZHANG, Jianwen GUO, Weiping GAO. Biosynthesis of elastin-like polypeptides and their applications in drug delivery[J]. Synthetic Biology Journal, 2022, 3(4): 728-747.
杨兆颖, 张帆, 郭建文, 高卫平. 类弹性蛋白多肽的生物合成及其药物递送应用[J]. 合成生物学, 2022, 3(4): 728-747.
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URL: https://synbioj.cip.com.cn/EN/10.12211/2096-8280.2021-094
Fig. 3 ELPs applications in extending half-life of drugs(a) Chemically conjugated ELP to small molecule drugs by inclusion of an intervening cleavable linker which releases the free drug intracellularly after endocytic uptake and accumulation in the acidic, enzyme-rich environment of endosomes and lysosomes; (b) Enhanced cellular uptake of ELP conjugates by functionalization of ELP with cell-penetrating peptides; (c) Schematic of ELP genetically fused to peptide and protein drugs; (d) Pharmacokinetics of NtTNF-VHHELP[43]; (e, f) Pharmacometabolic kinetics (e) and in vivo antitumor effificacy (f) after intravenous injections with IFNα-ELP and IFNα[44]
Fig. 4 Depot-forming ELP for drug delivery(a, b) Drugs form a subcutaneous insoluble coacervate upon injection and slowly dissolve from their surface to their core, steadily releasing the therapeutic into circulation; (c) The drug ELP conjugates can get into a tumor through the EPR effect and then be cleaved into free IFNα and ELP(V) by MMP-2 in the tumor, resulting in enhanced tumor penetration and antitumor efficacy[51]; (d) Fluorescence imaging of mice following MTD subcutaneous injection of Cy5-labeled IFNα-MMPS-ELP (V), IFN-MMPS-ELP (A), IFN-ELP (V), and IFNα; (e,f) pharmacokinetics (e) and antitumor efficacy (f) of mice after subcutaneous injections of IFNα-MMPS-ELP(V)、IFNα-MMPS-ELP(A)、IFNα-ELP(V) and IFNα at their MTDs.
Fig. 6 Schematic of ELPs in thermal targeting(The tumor tissue was locally heated, and the protein-ELP conjugates were phase transformed and enriched at the heating site.)
Fig. 7 (a) ELP micelles formed by diblock ELP, at a temperature between the Tt of the more hydrophobic ELP block (green, Tt 2) and the more hydrophilic ELP (blue, Tt1), the more hydrophobic block transitions and aggregates while the more hydrophilic block remains soluble, leading to self-assembly into micelles; (b) Cryo-TEM micrograph of ELP micelles[70]; (c,d) Pharmacokinetics (c) and antitumor efficacy (d) after intravenous injections with IFNα-ELPdiblock, IFNα-ELP(A), PEGASYS and IFNα [73]
Fig. 9 (a) Schematic of ELP hybrid nanoparticles loading hydrophobic drug molecules. Hydrophobic drugs are loaded in the core; (b) Synthetic route of LHRH-ELP-DOX nanoconjugates; (c) Cryo-TEM images of LHRH-ELP2-DOX and ELP2-DOX; (d) Pharmacokinetics of LHRH-ELP2-DOX in a DOX-resistant breast tumor mouse model; (e) Tumor volume changes after LHRH-ELP-DOX plus HIFU treatment [78]
Fig. 10 (a) Schematic of ELP hybrid nanoparticles loading hydrophilic drug molecules where hydrophobic sequences act as the core of the assembly when assembled and hydrophilic drugs are loaded within the hydrophobic core; (b) Cryo-TEM micrograph of ATBP-GEM conjugate; (c) AFM image of ATBP-GEM nanoparticles; (d,e) ATBP-GEM delayed the tumor growth (d) and improved the cumulative survival (e) of mice [80]
药物递送策略 | 应用 | ELP药物 | ELP序列信息 | 文献 |
---|---|---|---|---|
延长药物体内循环半衰期 | 髓样乳腺癌 | SynB1-ELP-DOX | (VPGXG)150 X=V5G3A2 | [ |
感染性休克 | NtTNF-VHHELP | (VPGXG)100 X=V5G3A2 | [ | |
淋巴瘤 | IFN-ELP | (VPGXG)90 X=V5G3A2 | [ | |
药物储库 | 2型糖尿病 | (GLP-1)-ELP | (GVGVP)120 | [ |
卵巢癌和黑色素瘤 | IFN-ELP | (VPGVG)90 | [ | |
胶质母细胞瘤 | IFN-ELP和替莫唑胺联合用药 | (VPGVG)90 | [ | |
黑色素瘤和卵巢癌 | IFN-MMPS-ELP | (VPGVG)90 | [ | |
创伤后关节炎 | xELP[IL-1Ra] | VPGKG(VPGVG)16~102 | [ | |
神经炎症 | ELP-curcumin | [VPGXG] L=60,80,160; X = V/I/E [1∶3∶1] | [ | |
骨损伤 | rhBMP-2-ELP | (VPGVG)40[(VPGVG)2(VPGCG)(VPGVG)2]2 | [ | |
热靶向治疗 | 卵巢癌、宫颈癌、人源咽鳞癌 | ELP1 | (VPGXG)150 X=V5G3A2 | [ |
两亲性自组装体 | 卵巢癌 | IFNα-ELPdiblock | ELP(A)48-ELP(V)48 | [ |
乳腺癌 | FKBP-ELP | G(Val-Pro-Gly-Ile-Gly)48(Val-Pro-Gly-Ser-Gly)48Y | [ | |
混合组装体 | 乳腺癌 | LHRH-ELP-DOX | (VPGXG)160 X=V1A8G7 | [ |
结肠癌 | ELP-(YG)6-(CGG)8-GEM | (VPGAG)160 | [ | |
乳腺癌 | DOXENC,M-ELP90A,120 | (VPGXG)120 X=A9V1 | [ | |
黑色素瘤 | DOX/PPy-ELP-F3 | (XGVPG)160 | [ | |
黑色素瘤、颈瘤、膀胱癌 | ELP-AuNP | (VPGVG)60 | [ | |
ELP水凝胶 | 骨再生 | CDEc | (VPGVG)120 | [ |
Tab. 1 Applications of ELP in drug delivery
药物递送策略 | 应用 | ELP药物 | ELP序列信息 | 文献 |
---|---|---|---|---|
延长药物体内循环半衰期 | 髓样乳腺癌 | SynB1-ELP-DOX | (VPGXG)150 X=V5G3A2 | [ |
感染性休克 | NtTNF-VHHELP | (VPGXG)100 X=V5G3A2 | [ | |
淋巴瘤 | IFN-ELP | (VPGXG)90 X=V5G3A2 | [ | |
药物储库 | 2型糖尿病 | (GLP-1)-ELP | (GVGVP)120 | [ |
卵巢癌和黑色素瘤 | IFN-ELP | (VPGVG)90 | [ | |
胶质母细胞瘤 | IFN-ELP和替莫唑胺联合用药 | (VPGVG)90 | [ | |
黑色素瘤和卵巢癌 | IFN-MMPS-ELP | (VPGVG)90 | [ | |
创伤后关节炎 | xELP[IL-1Ra] | VPGKG(VPGVG)16~102 | [ | |
神经炎症 | ELP-curcumin | [VPGXG] L=60,80,160; X = V/I/E [1∶3∶1] | [ | |
骨损伤 | rhBMP-2-ELP | (VPGVG)40[(VPGVG)2(VPGCG)(VPGVG)2]2 | [ | |
热靶向治疗 | 卵巢癌、宫颈癌、人源咽鳞癌 | ELP1 | (VPGXG)150 X=V5G3A2 | [ |
两亲性自组装体 | 卵巢癌 | IFNα-ELPdiblock | ELP(A)48-ELP(V)48 | [ |
乳腺癌 | FKBP-ELP | G(Val-Pro-Gly-Ile-Gly)48(Val-Pro-Gly-Ser-Gly)48Y | [ | |
混合组装体 | 乳腺癌 | LHRH-ELP-DOX | (VPGXG)160 X=V1A8G7 | [ |
结肠癌 | ELP-(YG)6-(CGG)8-GEM | (VPGAG)160 | [ | |
乳腺癌 | DOXENC,M-ELP90A,120 | (VPGXG)120 X=A9V1 | [ | |
黑色素瘤 | DOX/PPy-ELP-F3 | (XGVPG)160 | [ | |
黑色素瘤、颈瘤、膀胱癌 | ELP-AuNP | (VPGVG)60 | [ | |
ELP水凝胶 | 骨再生 | CDEc | (VPGVG)120 | [ |
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