Research Progress in Plant-Derived Vaccines Synthetic Biology Journal    DOI: 10.12211/2096-8280.2025-029

Fig. 3 The Mechanism of Action of P19 Protein
(The viral RNA that invades the plant body is assembled into double-stranded shRNA, which is cut into 21-23 base pair siRNAs by the Dicer enzyme. These siRNAs form a siRNA-RISC complex and bind to the homologous mRNA, causing it to be cleaved. However, when the P19 protein is expressed, it assembles into a dimer that binds to the siRNA, preventing the siRNA from binding to RISC, and thus the silencing process cannot proceed. By Figdraw.)

Other Images/Table from this Article

• Table 1  Plant-derived foreign substances that have been put into clinical or animal experiments and their expression methods
• Table 2  Plant-derived antigen examples
• Table 3  Examples of some plant-derived enveloped VLPs
• Fig. 1  The production process of plant-derived vaccines(Design and construct the plasmid vector for the gene. Introduce the vector into the host plant using appropriate methods. Allow the target gene to be expressed within the host plant. Collect the plant tissues that express the target protein. Grind the tissues to extract the protein. Purify the protein to obtain the final product.)
• Fig. 2  Optimization of Plant-derived Vaccines(The optimization of plant-derived vaccines can be achieved through the optimization of vectors, the selection of target antigens, the modification of proteins, co-expression of the target gene with protease inhibitor genes and silencing suppressor genes, and the optimization of the purification process. The co-expression of the P19 protein gene in the figure refers to the study by Jiexue et al. By Figdraw.)
• Table 4  Some commonly used plant protease inhibitors