合成生物学 ›› 2021, Vol. 2 ›› Issue (5): 716-733.doi: 10.12211/2096-8280.2021-058
林芝, 胡致伟, 瞿旭东, 林双君
收稿日期:
2021-05-07
修回日期:
2021-06-10
出版日期:
2021-10-31
发布日期:
2021-11-19
通讯作者:
林双君
作者简介:
基金资助:
Zhi LIN, Zhiwei HU, Xudong QU, Shuangjun LIN
Received:
2021-05-07
Revised:
2021-06-10
Online:
2021-10-31
Published:
2021-11-19
Contact:
Shuangjun LIN
摘要:
微生物发酵是一种经济高效、可持续的生产方式,可替代植物种植和化学合成来生产多种植物来源的药物。苄基异喹啉类生物碱作为植物来源生物碱的典型代表,具有多种重要的生理活性,已成为极具吸引力的微生物合成研究的靶标分子。随着该类生物碱天然生物合成途径逐渐被阐明以及多种酶学元件的发现,使得通过大肠杆菌和酿酒酵母等微生物宿主合成苄基异喹啉类生物碱的研究取得了重大进展。本综述着重介绍了这些进展中的突破性成果,包括苄基异喹啉类生物碱微生物合成过程中瓶颈反应的突破以及合成途径中相关酶的催化特性对代谢流的影响等,指出了微生物生产苄基异喹啉类生物碱走向工业化应用所面临的挑战,以及酶工程和新人工微生物合成途径的设计开发对克服这些挑战的重要性。
中图分类号:
林芝, 胡致伟, 瞿旭东, 林双君. 苄基异喹啉类生物碱的微生物合成研究进展及挑战[J]. 合成生物学, 2021, 2(5): 716-733, doi: 10.12211/2096-8280.2021-058.
Zhi LIN, Zhiwei HU, Xudong QU, Shuangjun LIN. Advances and challenges in microbial production of benzylisoquinoline alkaloids[J]. Synthetic Biology Journal, 2021, 2(5): 716-733, doi: 10.12211/2096-8280.2021-058.
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