合成生物学 ›› 2021, Vol. 2 ›› Issue (3): 354-370.DOI: 10.12211/2096-8280.2020-089
闫汉, 肖鹏峰, 刘全俊, 陆祖宏
收稿日期:
2020-12-21
修回日期:
2021-04-09
出版日期:
2021-06-30
发布日期:
2021-07-13
通讯作者:
刘全俊,陆祖宏
作者简介:
基金资助:
Han YAN, Pengfeng XIAO, Quanjun LIU, Zuhong LU
Received:
2020-12-21
Revised:
2021-04-09
Online:
2021-06-30
Published:
2021-07-13
Contact:
Quanjun LIU,Zuhong LU
摘要:
高通量、快速、低成本DNA合成是合成生物学、DNA信息存储以及DNA芯片等前沿科技领域的重要核心技术。DNA微阵列原位化学合成方法是在亚磷酸酰胺固相化学合成原理的基础上,整合了微电子学、计算科学、分子生物学、光电化学和微纳加工等学科的相关技术,近30年来得到了迅速的发展和应用。DNA微阵列原位化学合成方法根据不同的碱基分配方式可以分为原位光刻法、光敏抗蚀层合成法、光致酸法、喷印合成法、软光刻合成法、电致酸法和压印法以及以这些技术为基础衍生的各种合成方法等。本文对上述不同的DNA微阵列原位化学合成方法及其技术特点进行阐述,并对未来DNA合成方法的发展趋势进行讨论和展望。合成通量和效率方面基于CMOS芯片的电致酸DNA原位化学合成技术在未来10年内将具备较大的发展空间,通过解决芯片上微电极间氢离子串扰问题,有望实现单片TB级的DNA快速低成本合成。
中图分类号:
闫汉, 肖鹏峰, 刘全俊, 陆祖宏. DNA微阵列原位化学合成[J]. 合成生物学, 2021, 2(3): 354-370.
Han YAN, Pengfeng XIAO, Quanjun LIU, Zuhong LU. In situ chemical synthesis of DNA microarrays[J]. Synthetic Biology Journal, 2021, 2(3): 354-370.
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