Synthetic Biology Journal ›› 2024, Vol. 5 ›› Issue (1): 202-216.DOI: 10.12211/2096-8280.2022-068
• Invited Review • Previous Articles
Yao DU1,2, Hongdan GAO1,2, Jiakun LIU3,4, Xiaorong LIU2, Zhihao XING2, Tao ZHANG5, Dongli MA2
Received:
2022-11-26
Revised:
2023-02-15
Online:
2024-03-20
Published:
2024-02-29
Contact:
Dongli MA
杜瑶1,2, 高宏丹1,2, 刘家坤3,4, 刘孝荣2, 邢志浩2, 张涛5, 马东礼2
通讯作者:
马东礼
作者简介:
基金资助:
CLC Number:
Yao DU, Hongdan GAO, Jiakun LIU, Xiaorong LIU, Zhihao XING, Tao ZHANG, Dongli MA. Research progress of the CRISPR-Cas system in the detecting pathogen nucleic acids[J]. Synthetic Biology Journal, 2024, 5(1): 202-216.
杜瑶, 高宏丹, 刘家坤, 刘孝荣, 邢志浩, 张涛, 马东礼. CRISPR-Cas系统在病原核酸检测中的研究进展[J]. 合成生物学, 2024, 5(1): 202-216.
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URL: https://synbioj.cip.com.cn/EN/10.12211/2096-8280.2022-068
CRISPR类型 | 技术名称 | 效应 蛋白 | 目标 分子 | 扩增 方式 | 检测线 | 病原体 | 检测技术 |
---|---|---|---|---|---|---|---|
Type Ⅰ-E | CONAN[ | Cas3 | DNA | RT-LAMP | 1 copy | SARS-CoV-2 IAV | 侧向流动分析 |
Type Ⅴ | DETECTR[ | Cas12a | DNA | RPA | amol/L | HPV16/18 SARS-CoV-2 | 荧光信号 |
OR-DETECTR[ | Cas12a | RNA | RT-RPA | 1~2,5 copies/μL | SARS-CoV-2 H1N1 | 荧光信号、 侧向流动分析 | |
HOLMES[ | Cas12a | DNA RNA | PCR | amol/L | 日本脑炎病毒 伪狂犬病毒 | 荧光信号 | |
HOLMESv2[ | Cas12b | DNA RNA | LAMP | amol/L | 日本脑炎病毒 | 荧光信号 | |
CDetection[ | AaCas12b | DNA | RPA | nmol/L | HPV16/18 | 荧光信号 | |
E-CRISPR[ | Cas12a | DNA | — | pmol/L | HPV1、B19 | 电化学 | |
MoECS[ | Cas12 | DNA | — | fmol/L | SARS-CoV-2 Delta 变异株 | 电化学 | |
CRISPR-ENHANCE[ | LbCas12a | RNA | RT-LAMP | SARS-CoV-2、HIV、HCV | 侧向流动分析 | ||
AIOD-CRISPR[ | LbaCas12a | RNA | RPA | 11 copies | SARS-CoV-2 HIV-1 | 荧光信号、 视觉法 | |
SCAN[ | Cas12a | DNA RNA | RT-PCR | 13.5 copies/μL | SARS-CoV-2 HIV-1 | 纳米孔传感器 | |
TB-QUICK[ | Cas12b | DNA | LAMP | 1.3 copies/μL | 结核分枝杆菌 | 荧光信号 | |
STOPCovid[ | Cas12 | DNA RNA | LAMP | 100 copies | SARS-CoV-2 | 荧光信号 | |
STOPCovid.v2[ | Cas12 | DNA RNA | LAMP | 2000 copies/mL | SARS-CoV-2 | 荧光信号 | |
sPAMC[ | LbCas12a | DNA RNA | RPA | 1 copy/μL | SARS-CoV-2 | 荧光信号、 侧向流动分析 | |
Cas14-DETECTR[ | Cas14a | DNA | RPA | fmol/L | HBoV-1 | 荧光信号 | |
ACasB[ | Cas14a1 | DNA | — | 400 CFU/mL | 金黄色葡萄球菌 | 荧光信号 | |
Type Ⅵ | SHERLOCK[ | Cas13a | DNA RNA | RPA | amol/L | 病毒、细菌 | 荧光信号 |
HUDSON[ | Cas13a | RNA | RT-RPA | 1copy/μL | 寨卡病毒、登革病毒 | 荧光信号、 侧向流动分析 | |
OR-SHERLOCK[ | Cas13a | RNA | RT-RPA | 1~2 copies/μL, 5 copies/μL | SARS-CoV-2 H1N1 | 荧光信号、 侧向流动分析 | |
SHINE[ | Cas13 | RNA | RT-RPA | 10 copies/μL | SARS-CoV-2 | 智能手机(管内荧光读数或侧向流动分析) | |
FIND-IT[ | Lbu Cas13a | RNA | — | 31 copies/μL | SARS-CoV-2 | 荧光信号 (集成检测器) | |
CARMEN[ | LwCas13a | DNA RNA | PCR或RPA | amol/L | 169种人类感染病毒 | 荧光信号 | |
mCARMEN[ | LwCas13a | DNA RNA | PCR或RPA | 102 copies/μL | 21种人类呼吸道病毒 | 荧光信号 | |
Type Ⅴ Type Ⅵ Type Ⅲ | SHERLOCKv2[ | Cas13 Cas12a Csm6 | DNA RNA | RPA | zmol/L | 病毒、细菌 | 荧光信号、 侧向流动分析 |
Table 1 Technologies for detecting pathogens based on the CRISPR-Cas system
CRISPR类型 | 技术名称 | 效应 蛋白 | 目标 分子 | 扩增 方式 | 检测线 | 病原体 | 检测技术 |
---|---|---|---|---|---|---|---|
Type Ⅰ-E | CONAN[ | Cas3 | DNA | RT-LAMP | 1 copy | SARS-CoV-2 IAV | 侧向流动分析 |
Type Ⅴ | DETECTR[ | Cas12a | DNA | RPA | amol/L | HPV16/18 SARS-CoV-2 | 荧光信号 |
OR-DETECTR[ | Cas12a | RNA | RT-RPA | 1~2,5 copies/μL | SARS-CoV-2 H1N1 | 荧光信号、 侧向流动分析 | |
HOLMES[ | Cas12a | DNA RNA | PCR | amol/L | 日本脑炎病毒 伪狂犬病毒 | 荧光信号 | |
HOLMESv2[ | Cas12b | DNA RNA | LAMP | amol/L | 日本脑炎病毒 | 荧光信号 | |
CDetection[ | AaCas12b | DNA | RPA | nmol/L | HPV16/18 | 荧光信号 | |
E-CRISPR[ | Cas12a | DNA | — | pmol/L | HPV1、B19 | 电化学 | |
MoECS[ | Cas12 | DNA | — | fmol/L | SARS-CoV-2 Delta 变异株 | 电化学 | |
CRISPR-ENHANCE[ | LbCas12a | RNA | RT-LAMP | SARS-CoV-2、HIV、HCV | 侧向流动分析 | ||
AIOD-CRISPR[ | LbaCas12a | RNA | RPA | 11 copies | SARS-CoV-2 HIV-1 | 荧光信号、 视觉法 | |
SCAN[ | Cas12a | DNA RNA | RT-PCR | 13.5 copies/μL | SARS-CoV-2 HIV-1 | 纳米孔传感器 | |
TB-QUICK[ | Cas12b | DNA | LAMP | 1.3 copies/μL | 结核分枝杆菌 | 荧光信号 | |
STOPCovid[ | Cas12 | DNA RNA | LAMP | 100 copies | SARS-CoV-2 | 荧光信号 | |
STOPCovid.v2[ | Cas12 | DNA RNA | LAMP | 2000 copies/mL | SARS-CoV-2 | 荧光信号 | |
sPAMC[ | LbCas12a | DNA RNA | RPA | 1 copy/μL | SARS-CoV-2 | 荧光信号、 侧向流动分析 | |
Cas14-DETECTR[ | Cas14a | DNA | RPA | fmol/L | HBoV-1 | 荧光信号 | |
ACasB[ | Cas14a1 | DNA | — | 400 CFU/mL | 金黄色葡萄球菌 | 荧光信号 | |
Type Ⅵ | SHERLOCK[ | Cas13a | DNA RNA | RPA | amol/L | 病毒、细菌 | 荧光信号 |
HUDSON[ | Cas13a | RNA | RT-RPA | 1copy/μL | 寨卡病毒、登革病毒 | 荧光信号、 侧向流动分析 | |
OR-SHERLOCK[ | Cas13a | RNA | RT-RPA | 1~2 copies/μL, 5 copies/μL | SARS-CoV-2 H1N1 | 荧光信号、 侧向流动分析 | |
SHINE[ | Cas13 | RNA | RT-RPA | 10 copies/μL | SARS-CoV-2 | 智能手机(管内荧光读数或侧向流动分析) | |
FIND-IT[ | Lbu Cas13a | RNA | — | 31 copies/μL | SARS-CoV-2 | 荧光信号 (集成检测器) | |
CARMEN[ | LwCas13a | DNA RNA | PCR或RPA | amol/L | 169种人类感染病毒 | 荧光信号 | |
mCARMEN[ | LwCas13a | DNA RNA | PCR或RPA | 102 copies/μL | 21种人类呼吸道病毒 | 荧光信号 | |
Type Ⅴ Type Ⅵ Type Ⅲ | SHERLOCKv2[ | Cas13 Cas12a Csm6 | DNA RNA | RPA | zmol/L | 病毒、细菌 | 荧光信号、 侧向流动分析 |
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