Synthetic Biology Journal ›› 2022, Vol. 3 ›› Issue (2): 302-319.DOI: 10.12211/2096-8280.2021-063
• Invited Review • Previous Articles Next Articles
Qian SHI, Yuanyuan WU, yang YANG
Received:
2021-06-04
Revised:
2021-10-24
Online:
2022-05-11
Published:
2022-04-30
Contact:
yang YANG
施茜, 吴园园, 杨洋
通讯作者:
杨洋
作者简介:
基金资助:
CLC Number:
Qian SHI, Yuanyuan WU, yang YANG. DNA nanotechnology and synthetic biology[J]. Synthetic Biology Journal, 2022, 3(2): 302-319.
施茜, 吴园园, 杨洋. DNA纳米技术与合成生物学[J]. 合成生物学, 2022, 3(2): 302-319.
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URL: https://synbioj.cip.com.cn/EN/10.12211/2096-8280.2021-063
Fig. 2 Basic principle of DNA origami and its development (Scale bar: 50 nm)(a) Principle for the 2D assembly of DNA origami[9]; (b) 3D structure assembly with the bending control[11]; (c) Design for curved surface and DNA gridiron[12-13]
根据结合方式分类 | DNA修饰 | 蛋白修饰 | 优点 | 缺点 | |
---|---|---|---|---|---|
非共价偶联 | 生物素-链亲和素[ | 生物素 | 链亲和素 | ①具有可逆性; | 结合不稳定 |
Ni-NTA-Histag[ | NTA | Histag | ②可进行定点定位修饰 | ||
抗体-抗原[ | 抗原 | 抗体 | |||
核酸适配体-蛋白[ | 核酸适配体 | — | |||
DNA结合蛋白[ | 特异的dsDNA | 锌指蛋白 | |||
RNA-病毒蛋白[ | RNA | 病毒蛋白 | |||
DNA-衣壳蛋白[ | — | 衣壳蛋白 | |||
共价偶联 | √非特异性 | ①结合较稳定; | 结合位点不易控制 | ||
SPDP[ | 氨基 | 半胱氨酸残基 | ②反应温和,步骤简单 | ||
Sulfo-SMCC[ | 氨基 | 半胱氨酸残基 | |||
√特异性 | |||||
SNAP-tag[ | O6-烷基鸟嘌呤 | SNAP-tag | ①结合较稳定; | 需要进行蛋白质工程 | |
Halo-tag[ | 5-氯已烷 | Halo-tag | ②可控制结合位点 | 操作较复杂 |
Tab. 1 Classification of protein assembly
根据结合方式分类 | DNA修饰 | 蛋白修饰 | 优点 | 缺点 | |
---|---|---|---|---|---|
非共价偶联 | 生物素-链亲和素[ | 生物素 | 链亲和素 | ①具有可逆性; | 结合不稳定 |
Ni-NTA-Histag[ | NTA | Histag | ②可进行定点定位修饰 | ||
抗体-抗原[ | 抗原 | 抗体 | |||
核酸适配体-蛋白[ | 核酸适配体 | — | |||
DNA结合蛋白[ | 特异的dsDNA | 锌指蛋白 | |||
RNA-病毒蛋白[ | RNA | 病毒蛋白 | |||
DNA-衣壳蛋白[ | — | 衣壳蛋白 | |||
共价偶联 | √非特异性 | ①结合较稳定; | 结合位点不易控制 | ||
SPDP[ | 氨基 | 半胱氨酸残基 | ②反应温和,步骤简单 | ||
Sulfo-SMCC[ | 氨基 | 半胱氨酸残基 | |||
√特异性 | |||||
SNAP-tag[ | O6-烷基鸟嘌呤 | SNAP-tag | ①结合较稳定; | 需要进行蛋白质工程 | |
Halo-tag[ | 5-氯已烷 | Halo-tag | ②可控制结合位点 | 操作较复杂 |
Fig. 4 DNA nanoframe-directed assembly of proteins and phospholipids(a) DNA swing arm and regulation on cascade enzymatic reactions[61]; (b) Vesicles with controllable sizes [62]; (c) Two-dimensional lipid bilayer assembly[64]
Fig. 5 Mimic and construction of cell elements(a) Simulation of the nuclear pore complex [55]; (b) Simulation of cytoskeleton protein and network [70]
Fig. 6 Mimic king biological reactions and biochemical systems(a) Simulation of SNAREs protein-induced membrane fusion[71]; (b) Mimicking BAR protein-induced membrane tubulation [74]; (c) Producing RNA in a nanofactory[75]; (d) Assembly TMV capsid protein on the DNA template [52]; (e) Autonomous regulation of the thrombin dependent coagulation [77]; (f) Simulation of the G-actin movement [78]
Fig. 7 Applications in biomedicines(a) Logic-gate controlled DNA nanorobot[81]; (b) Viral capsid protein modified DNA nanostructure[104]; (c) Virus-inspired membrane-coated DNA nanostructure[105]
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