Synthetic Biology Journal ›› 2022, Vol. 3 ›› Issue (3): 465-486.DOI: 10.12211/2096-8280.2021-064
• Invited Review • Previous Articles Next Articles
Jiaqi HOU1,2, Nan JIANG1,2, Lianju MA2, Yuan LU1
Received:
2021-06-06
Revised:
2021-09-21
Online:
2022-07-13
Published:
2022-06-30
Contact:
Lianju MA, Yuan LU
后佳琦1,2, 姜楠1,2, 马莲菊2, 卢元1
通讯作者:
马莲菊,卢元
作者简介:
基金资助:
CLC Number:
Jiaqi HOU, Nan JIANG, Lianju MA, Yuan LU. Cell-free protein synthesis: from basic research to engineering applications[J]. Synthetic Biology Journal, 2022, 3(3): 465-486.
后佳琦, 姜楠, 马莲菊, 卢元. 无细胞蛋白质合成:从基础研究到工程应用[J]. 合成生物学, 2022, 3(3): 465-486.
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URL: https://synbioj.cip.com.cn/EN/10.12211/2096-8280.2021-064
Fig. 2 Comparison of protein synthesis through in vivo cellular and in vitro cell-free systems(In the in vivo cellular system, the target plasmid is introduced into the cell, and the target protein is obtained through cell culture, lysis, and product purification. In the CFPS system, by adding DNA template, amino acids, NTPs, energy substrates and other cofactors to the cell extract, the target protein is obtained after centrifugation and purification.)
Fig.3 Cell-free protein synthesis process(The components of the cell-free system include cell extracts, cofactors, DNA templates, etc. Through the transcription and translation machinery in the cell extracts, the cell-free system can produce the target protein after incubating for several hours under designated conditions.)
细胞提取物类型 | 优点 | 缺点 |
---|---|---|
大肠杆菌细胞 | 生长速度快、易于培养、产量高、成本效益高 | 在翻译后修饰、膜蛋白合成和其他难以合成的蛋白质合成方面存在局限性 |
酿酒酵母细胞 | 易于培养、可用于研究真核生物的转录翻译机制 | 蛋白质产量低,未显示充分的翻译后修饰 |
小麦胚芽细胞 | 蛋白质可溶性增强、可表达毒性蛋白质、产量高 | 需要去除各种抑制酶,操作流程较繁琐,合成复杂蛋白、进行翻译后修饰具有局限性 |
烟草细胞 | 有利于合成翻译后修饰蛋白质,还有助于糖基化和二硫 键形成,相对其他真核平台耗时短 | 蛋白质产量一般,未显示充分的翻译后修饰 |
兔网织红细胞 | 具有翻译后修饰功能,可表达膜蛋白、毒性蛋白 | 蛋白质产量低,耗时长 |
中国仓鼠卵巢细胞 | 具有翻译后修饰功能,可表达膜蛋白、毒性蛋白 | 蛋白质产量较低 |
昆虫细胞 | 具有翻译后修饰功能,可表达膜蛋白、冰结构蛋白 | 蛋白质产量较低,无细胞体系中需要更多的提取物 |
PURE | 组分纯化后无核酸酶或蛋白酶残留,灵活和模块化 | 成本较高,不能激活内源性代谢 |
Tab. 1 Comparison of advantages and disadvantages of different extract systems
细胞提取物类型 | 优点 | 缺点 |
---|---|---|
大肠杆菌细胞 | 生长速度快、易于培养、产量高、成本效益高 | 在翻译后修饰、膜蛋白合成和其他难以合成的蛋白质合成方面存在局限性 |
酿酒酵母细胞 | 易于培养、可用于研究真核生物的转录翻译机制 | 蛋白质产量低,未显示充分的翻译后修饰 |
小麦胚芽细胞 | 蛋白质可溶性增强、可表达毒性蛋白质、产量高 | 需要去除各种抑制酶,操作流程较繁琐,合成复杂蛋白、进行翻译后修饰具有局限性 |
烟草细胞 | 有利于合成翻译后修饰蛋白质,还有助于糖基化和二硫 键形成,相对其他真核平台耗时短 | 蛋白质产量一般,未显示充分的翻译后修饰 |
兔网织红细胞 | 具有翻译后修饰功能,可表达膜蛋白、毒性蛋白 | 蛋白质产量低,耗时长 |
中国仓鼠卵巢细胞 | 具有翻译后修饰功能,可表达膜蛋白、毒性蛋白 | 蛋白质产量较低 |
昆虫细胞 | 具有翻译后修饰功能,可表达膜蛋白、冰结构蛋白 | 蛋白质产量较低,无细胞体系中需要更多的提取物 |
PURE | 组分纯化后无核酸酶或蛋白酶残留,灵活和模块化 | 成本较高,不能激活内源性代谢 |
Fig. 4 Modes for CFPS operation(a) Batch mode. All reaction components are added to one tube, which is simple and convenient for operating. (b) Continuous exchange. Through the semi-permeable membrane, nutrients are separated from the metabolic by-products, which overcomes the inhibitory effect of the metabolic products on the reaction. (c) Tube in tube. The gas is continuously pumped into the inner tube through the air pump, which significantly improves the efficiency of oxygen transfer and increases the output. (d) Digital microfluidic technology. It can control the movement, mixing, and separation of a single droplet, with good interactivity and flexibility.
Fig. 5 Prototype designs for genetic circuits(The transcription unit is encoded by a single plasmid or linear DNA template, which can be used as an analog of a logic gate, and then molecular calculations or genetic programs are performed in vitro through a cell-free gene expression reaction to predict the function of the circuit in the cell.)
Fig. 6 Applications of cell-free protein engineering(The applications of cell-free protein engineering mainly include membrane proteins, virus-like particles, post-translational modification, unnatural amino acid intercalation, and protein evolution)
Fig. 7 Construction of artificial cells(Molecular crowding, compartmentalization, gene noise, networks, dynamic behaviors and cell communications are the structural and functional characteristics necessary to maintain the normal life activities of a cell, which can effectively regulate and operate the cell)
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