Synthetic Biology Journal ›› 2023, Vol. 4 ›› Issue (2): 318-332.doi: 10.12211/2096-8280.2022-061

• Invited Review • Previous Articles     Next Articles

Synthetic biology for next-generation genetic diagnostics

Hailong LV1,2,3, Jian WANG2, Hao LV4, Jin WANG2,5, Yong XU3, Dayong GU3   

  1. 1.Guangdong Key Laboratory for Biomedical Measurements and Ultrasound Imaging. School of Biomedical Engineering,School of Medicine,Shenzhen University,Shenzhen 518060,Guangdong,China
    2.Shenzhen Institute of Translational Medicine,Health Science Center,The First Affiliated Hospital of Shenzhen University,Shenzhen Second People's Hospital,Shenzhen 518035,Guangdong,China
    3.Department of Laboratory Medicine,The First Affiliated Hospital of Shenzhen University,Shenzhen Second People's Hospital,Shenzhen 518035,Guangdong,China
    4.Department of Neurosurgery,Shenzhen Key Laboratory of Neurosurgery,the Shenzhen Second People’s Hospital,First Affiliated Hospital of Shenzhen University,Shenzhen 518037,Guangdong,China
    5.Shanghai Tolo Biotechnology Co. ,Ltd. ,Shanghai 200233,China
  • Received:2022-11-02 Revised:2022-12-17 Online:2023-04-30 Published:2023-04-27
  • Contact: Dayong GU


In recent years, rapid advances have been made for synthetic biology technologies, which contribute greatly to technological innovations and applications in genetic diagnostics. Diagnostic methods for accurate detection of diseases and monitoring treatment responses are essential for effective clinical managements. Synthetic biology seeks to redesign biological systems to perform new functions in a predictable manner. A review on recent advances in synthetic biology as a diagnostic method for accurate detection of diseases and monitoring therapeutic responses is essential for effective clinical management, and also important for prevention, prediction and prognosis of diseases. In this article, we first describes the categories of synthetic biology applications in genetic diagnostics, including different biosensors that have been developed for both in vitro and in vivo monitoring. Subsequently, perspectives of the next generation of genetic diagnostic technologies and progress of synthetic biology devices and technologies in genetic diagnostics are highlighted. In addition, we further address the current technical challenges in genetic diagnostics and clinical applications. Finally, we draw attention to the latest innovations in synthetic biology that may have a significant impact on the future applications of genetic diagnostics.

Key words: synthetic biology, genetic diagnostics, CRISPR, translational medicine, biotechnology

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