Synthetic Biology Journal ›› 2024, Vol. 5 ›› Issue (5): 1211-1226.DOI: 10.12211/2096-8280.2024-024
• Invited Review • Previous Articles
Received:
2024-03-19
Revised:
2024-06-08
Online:
2024-11-20
Published:
2024-10-31
Contact:
Guo-Qiang CHEN
通讯作者:
陈国强
作者简介:
基金资助:
CLC Number:
Guo-Qiang CHEN, Dan TAN. Reprogramming microbial chassis for low-cost bioprodcution of tailor-made polyhydroxyalkanoates[J]. Synthetic Biology Journal, 2024, 5(5): 1211-1226.
陈国强, 谭丹. 重编程微生物底盘用于PHA材料的定制化低成本生物合成[J]. 合成生物学, 2024, 5(5): 1211-1226.
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URL: https://synbioj.cip.com.cn/EN/10.12211/2096-8280.2024-024
聚合物类型 Polymer type | 熔融温度(Tm)/℃ Melting temperature/℃ | 玻璃化转变温度(Tg)/℃ Glass transition temperature/℃ | 拉伸强度/MPa Tensile strength/MPa | 断裂伸长率/% Elongation at break/% |
---|---|---|---|---|
PHB | 178 | 4 | 43 | 5 |
P(3HB-20% 3HV) | 145 | -1 | 20 | 50 |
P(3HB-17% 3HHx) | 120 | -2 | 20 | 850 |
P(4HB) | 58 | -48 | 104 | 1000 |
P(3HB-45% 4HB) | 162 | -16 | 3 | 268 |
P(3HP) | 78.1 | -17.9 | 33.8 | 497.6 |
P(7% 3HHx-3HO) | 61 | -37.8 | 7.4 | 346.3 |
P(10% 3HHx-86% 3HO-4% 3HD) | 61 | -35 | 10 | 300 |
PP | 186 | -10 | 38 | 400 |
PET | 262 | — | 56 | 8300 |
HDPE | 135 | — | 29 | — |
Table 1 Comparison of material properties between typical PHAs and traditional plastics [12]
聚合物类型 Polymer type | 熔融温度(Tm)/℃ Melting temperature/℃ | 玻璃化转变温度(Tg)/℃ Glass transition temperature/℃ | 拉伸强度/MPa Tensile strength/MPa | 断裂伸长率/% Elongation at break/% |
---|---|---|---|---|
PHB | 178 | 4 | 43 | 5 |
P(3HB-20% 3HV) | 145 | -1 | 20 | 50 |
P(3HB-17% 3HHx) | 120 | -2 | 20 | 850 |
P(4HB) | 58 | -48 | 104 | 1000 |
P(3HB-45% 4HB) | 162 | -16 | 3 | 268 |
P(3HP) | 78.1 | -17.9 | 33.8 | 497.6 |
P(7% 3HHx-3HO) | 61 | -37.8 | 7.4 | 346.3 |
P(10% 3HHx-86% 3HO-4% 3HD) | 61 | -35 | 10 | 300 |
PP | 186 | -10 | 38 | 400 |
PET | 262 | — | 56 | 8300 |
HDPE | 135 | — | 29 | — |
Fig. 2 The major PHA biosynthetic pathways(PHA biosynthetic pathways fed with related and unrelated carbon sources in the three PHA classic pathways: glucose glycolysis pathway, β-oxidation pathway, and de novo synthesis pathway offatty acids)
生产菌 Producers | PHA类型 PHAs | 底物 Substrates | 细胞干重/(g/L) Cell dry weight/(g/L) | PHA质量 分数/% PHA content/% | 最高体积产率/[g/(L·h)] Highest volumetric productivity/[g/(L·h)] | 参考文献 References |
---|---|---|---|---|---|---|
Escherichia coli | Various PHAs | Glucose | 141.6 | 73 | 4.63 | [ |
Ralstonia eutropha | SCL-PHAs, MCL-PHAs, PHBHHx | Glucose Fatty acid | 232 | 80 | 3.14 | [ |
Aeromonas hydrophila | PHBHHx | Fatty acid | 43.3 | 45.2 | 1.01 | [ |
Pseudomonas spp. | MCL-PHAs | Fatty acid | 72.6 | 51.4 | 1.91 | [ |
Halomonas spp. | SCL-PHAs | Glucose | 100 | 60~92 | 1.67~3.2 | [ |
Table 2 Main PHA producers and their maximal PHA yields
生产菌 Producers | PHA类型 PHAs | 底物 Substrates | 细胞干重/(g/L) Cell dry weight/(g/L) | PHA质量 分数/% PHA content/% | 最高体积产率/[g/(L·h)] Highest volumetric productivity/[g/(L·h)] | 参考文献 References |
---|---|---|---|---|---|---|
Escherichia coli | Various PHAs | Glucose | 141.6 | 73 | 4.63 | [ |
Ralstonia eutropha | SCL-PHAs, MCL-PHAs, PHBHHx | Glucose Fatty acid | 232 | 80 | 3.14 | [ |
Aeromonas hydrophila | PHBHHx | Fatty acid | 43.3 | 45.2 | 1.01 | [ |
Pseudomonas spp. | MCL-PHAs | Fatty acid | 72.6 | 51.4 | 1.91 | [ |
Halomonas spp. | SCL-PHAs | Glucose | 100 | 60~92 | 1.67~3.2 | [ |
Fig. 3 Next Generation Industrial Biotechnology (NGIB) based on Halomonas spp.[80](An open/unsterile and continuous, energy and freshwater-saving bio-process for the production of various intracellular and extracellular products, which allows morphology engineering for the easy and economic downstream processing.)
公司 Companies | PHA类型 PHAs Types | 技术 Technology | 规模/(吨/年) Scale/(t/a) | 官方网站 Websites |
---|---|---|---|---|
Go!PHA, The Netherlands | All Types | PHA Global Promotion | Unknown | gopha.org |
PhaBuilder, China | All Types | Halomonas spp. (NGIB①) | 1000~10 000 | www.phabuilder.com |
Medpha, China | P3HB4HB | Halomonas spp. (NGIB①) | 100 | www.medpha.com.cn |
COFCO, China | PHB | Halomonas spp. (NGIB①) | 1000 | www.cofco.com |
Bluepha, China | PHBHHx | Ralstonia eutropha and NGIB | 1000 | www.bluepha.com |
TianAn Biopolymer, China | PHBV | Ralstonia eutropha | 2000 | www.tianan-enmat.com |
GreenBio, Tianjin, China | P3HB4HB | Escherichia coli | 10 000 | www.tjgreenbio.com |
Ecomann, Shenzhen, China | P3HB4HB | Escherichia coli | 10 000 | ecomannbruce.plasway.com |
RWDC, Singapore and USA | PHBHHx | Ralstonia eutropha | Unknown | www.rwdc-industries.com |
Danimer Scientific, USA | PHBHHx | Ralstonia eutropha | 10 000 | danimerscientific.com |
Full Cycle, USA | PHA② | non-GMO bacteria | Unknown | fullcyclebioplastics.com |
Newlight, USA | PHB | Ocean microbes grown on greenhouse gas | Unknown | www.newlight.com |
Metabolix, USA | P3HB4HB | Escherichia coli | 5000 | IP sold to CJ, Korea |
BOSK Bioproducts, Canada | PHA② | Forest wastes for PHA production | Unknown | www.bosk-bioproducts.com |
Genecis, Canada | PHBV | Unknown | Unknown | genecis.co |
TerraVerdae Bioworks,Canada | PHA② | Unknown | Unknown | terraverdae.com |
Kaneka, Japan | PHBHHx | Ralstonia eutropha | 5000 | www.kaneka.be |
Nafigate, France | PHB | Toxic waste as substrates | Unknown | www.nafigate.com |
CJ, Korea | P3HB4HB | Escherichia coli | Unknown | www.cj.co.kr |
Helian Polymers, The Netherlands | PHB/PHBV | non-GMO bacteria | Unknown | helianpolymers.com |
Biocycle, Brazil | PHB | Bacillus spp. | 100 | fapesp.br |
Biomer, Germany | PHB | Alcaligenes latus | Unknown | biomer.de |
Bioextrax, Sweden | PHA② | Bioextrax DSP method | Unknown | bioextrax.com |
SABIO srl, Italy | PHA② | Organic wastes for PHA production | Unknown | www.bio-on.it |
Table 3 PHAs commercialization companies[6]
公司 Companies | PHA类型 PHAs Types | 技术 Technology | 规模/(吨/年) Scale/(t/a) | 官方网站 Websites |
---|---|---|---|---|
Go!PHA, The Netherlands | All Types | PHA Global Promotion | Unknown | gopha.org |
PhaBuilder, China | All Types | Halomonas spp. (NGIB①) | 1000~10 000 | www.phabuilder.com |
Medpha, China | P3HB4HB | Halomonas spp. (NGIB①) | 100 | www.medpha.com.cn |
COFCO, China | PHB | Halomonas spp. (NGIB①) | 1000 | www.cofco.com |
Bluepha, China | PHBHHx | Ralstonia eutropha and NGIB | 1000 | www.bluepha.com |
TianAn Biopolymer, China | PHBV | Ralstonia eutropha | 2000 | www.tianan-enmat.com |
GreenBio, Tianjin, China | P3HB4HB | Escherichia coli | 10 000 | www.tjgreenbio.com |
Ecomann, Shenzhen, China | P3HB4HB | Escherichia coli | 10 000 | ecomannbruce.plasway.com |
RWDC, Singapore and USA | PHBHHx | Ralstonia eutropha | Unknown | www.rwdc-industries.com |
Danimer Scientific, USA | PHBHHx | Ralstonia eutropha | 10 000 | danimerscientific.com |
Full Cycle, USA | PHA② | non-GMO bacteria | Unknown | fullcyclebioplastics.com |
Newlight, USA | PHB | Ocean microbes grown on greenhouse gas | Unknown | www.newlight.com |
Metabolix, USA | P3HB4HB | Escherichia coli | 5000 | IP sold to CJ, Korea |
BOSK Bioproducts, Canada | PHA② | Forest wastes for PHA production | Unknown | www.bosk-bioproducts.com |
Genecis, Canada | PHBV | Unknown | Unknown | genecis.co |
TerraVerdae Bioworks,Canada | PHA② | Unknown | Unknown | terraverdae.com |
Kaneka, Japan | PHBHHx | Ralstonia eutropha | 5000 | www.kaneka.be |
Nafigate, France | PHB | Toxic waste as substrates | Unknown | www.nafigate.com |
CJ, Korea | P3HB4HB | Escherichia coli | Unknown | www.cj.co.kr |
Helian Polymers, The Netherlands | PHB/PHBV | non-GMO bacteria | Unknown | helianpolymers.com |
Biocycle, Brazil | PHB | Bacillus spp. | 100 | fapesp.br |
Biomer, Germany | PHB | Alcaligenes latus | Unknown | biomer.de |
Bioextrax, Sweden | PHA② | Bioextrax DSP method | Unknown | bioextrax.com |
SABIO srl, Italy | PHA② | Organic wastes for PHA production | Unknown | www.bio-on.it |
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