Guoqiang CHEN1, Dan TAN2
Received:
2024-03-19
Revised:
2024-06-08
Published:
2024-06-25
Contact:
Guoqiang CHEN
陈国强1, 谭丹2
通讯作者:
陈国强
作者简介:
基金资助:
CLC Number:
Guoqiang CHEN, Dan TAN. Reprogramming Microbial Chassis for Low-cost Bioprodcution of Tailor-made Polyhydroxyalkanoates[J]. Synthetic Biology Journal, DOI: 10.12211/2096-8280.2024-024.
陈国强, 谭丹. 重编程微生物底盘用于PHA材料的定制化低成本生物合成[J]. 合成生物学, DOI: 10.12211/2096-8280.2024-024.
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URL: https://synbioj.cip.com.cn/EN/10.12211/2096-8280.2024-024
聚合物类型 Polymer type | 熔点(Tm, ℃) Melting temperature (Tm, ℃) | 玻璃化转变温度(Tg, ℃) Glass transition temperature (Tg, ℃) | 抗拉强度(MPa) Tensile strength(MPa) | 断裂伸长率(%) Elongation at break(%) |
---|---|---|---|---|
PHB | 178 | 4 | 43 | 5 |
P(3HB-20 mol% 3HV) | 145 | -1 | 20 | 50 |
P(3HB-17 mol% 3HHx) | 120 | -2 | 20 | 850 |
P(4HB) | 58 | -48 | 104 | 1000 |
P(3HB-45 mol% 4HB) | 162 | -16 | 3 | 268 |
P(3HP) | 78.1 | -17.9 | 33.8 | 497.6 |
P(7 mol% 3HHx-3HO) | 61 | -37.8 | 7.4 | 346.3 |
P(10 mol% 3HHx-86 mol% 3HO-4 mol% 3HD) | 61 | -35 | 10 | 300 |
PP | 186 | -10 | 38 | 400 |
PET | 262 | - | 56 | 8300 |
HDPE | 135 | - | 29 | - |
Table 1 Comparison of material properties between typical PHAs and traditional plastics [15]
聚合物类型 Polymer type | 熔点(Tm, ℃) Melting temperature (Tm, ℃) | 玻璃化转变温度(Tg, ℃) Glass transition temperature (Tg, ℃) | 抗拉强度(MPa) Tensile strength(MPa) | 断裂伸长率(%) Elongation at break(%) |
---|---|---|---|---|
PHB | 178 | 4 | 43 | 5 |
P(3HB-20 mol% 3HV) | 145 | -1 | 20 | 50 |
P(3HB-17 mol% 3HHx) | 120 | -2 | 20 | 850 |
P(4HB) | 58 | -48 | 104 | 1000 |
P(3HB-45 mol% 4HB) | 162 | -16 | 3 | 268 |
P(3HP) | 78.1 | -17.9 | 33.8 | 497.6 |
P(7 mol% 3HHx-3HO) | 61 | -37.8 | 7.4 | 346.3 |
P(10 mol% 3HHx-86 mol% 3HO-4 mol% 3HD) | 61 | -35 | 10 | 300 |
PP | 186 | -10 | 38 | 400 |
PET | 262 | - | 56 | 8300 |
HDPE | 135 | - | 29 | - |
Fig. 2 The major PHA biosynthetic pathways(PHA biosynthetic pathways fed with related and unrelated carbon sources in the three PHA classic pathways: glucose glycolysis pathway, β-oxidation pathway, and de novo synthesis pathway offatty acids)
生产菌 Producers | PHA类型 PHAs | 底物 Substrates | 细胞干重(g/L) Cell dry weight (g/L) | PHA含量(wt%) PHA content (wt%) | 最高体积产率(g/L/h) Highest volumetric productivity (g/L/h) | 参考文献 References |
---|---|---|---|---|---|---|
Escherichia coli | Various PHAs | Glucose | 141.6 | 73 | 4.63 | [ |
Ralstonia eutropha | SCL-PHAs, MCL-PHAs, PHBHHx | Glucose Fatty acid | 232 | 80 | 3.14 | [ |
Aeromonas hydrophila | PHBHHx | Fatty acid | 43.3 | 45.2 | 1.01 | [ |
Pseudomonas spp. | MCL-PHAs | Fatty acid | 72.6 | 51.4 | 1.91 | [ |
Halomonas spp. | SCL-PHAs | Glucose | 100 | 60-92 | 1.67-3.2 | [ |
Table 2 Main PHA producers and their maximal PHA yields
生产菌 Producers | PHA类型 PHAs | 底物 Substrates | 细胞干重(g/L) Cell dry weight (g/L) | PHA含量(wt%) PHA content (wt%) | 最高体积产率(g/L/h) Highest volumetric productivity (g/L/h) | 参考文献 References |
---|---|---|---|---|---|---|
Escherichia coli | Various PHAs | Glucose | 141.6 | 73 | 4.63 | [ |
Ralstonia eutropha | SCL-PHAs, MCL-PHAs, PHBHHx | Glucose Fatty acid | 232 | 80 | 3.14 | [ |
Aeromonas hydrophila | PHBHHx | Fatty acid | 43.3 | 45.2 | 1.01 | [ |
Pseudomonas spp. | MCL-PHAs | Fatty acid | 72.6 | 51.4 | 1.91 | [ |
Halomonas spp. | SCL-PHAs | Glucose | 100 | 60-92 | 1.67-3.2 | [ |
Fig. 3 Next Generation Industrial Biotechnology (NGIB) based on Halomonas spp., an open/unsterile and continuous, energy and freshwater-saving bio-process for the production of various intracellular and extracellular products, which allows morphology engineering for the easy and economic downstream processing.
公司 Companies | PHA类型 PHAs Types | 技术 Technology | 规模(吨/年) Scale (ton/year) | 官方网站 Websites |
---|---|---|---|---|
Go!PHA, The Netherlands | All Types | PHA Global Promotion | Unknown | gopha.org |
PhaBuilder, China | All Types | Halomonas spp (NGIBa) | 1 000-10 000 | www.phabuilder.com |
Medpha, China | P3HB4HB | Halomonas spp (NGIBa) | 100 | www.medpha.com.cn |
COFCO, China | PHB | Halomonas spp (NGIBa) | 1 000 | www.cofco.com |
Bluepha, China | PHBHHx | Ralstonia eutropha and NGIB | 1 000 | www.bluepha.com |
TianAn Biopolymer, China | PHBV | Ralstonia eutropha | 2 000 | www.tianan-enmat.com |
GreenBio, Tianjin, China | P3HB4HB | Escherichia coli | 10000 | www.tjgreenbio.com |
Ecomann, Shenzhen, China | P3HB4HB | Escherichia coli | 10000 | ecomannbruce.plasway.com |
RWDC, Singapore and USA | PHBHHx | Ralstonia eutropha | Unknown | www.rwdc-industries.com |
Danimer Scientific, USA | PHBHHx | Ralstonia eutropha | 10 000 | danimerscientific.com |
Full Cycle, USA | PHAb | non-GMO bacteria | Unknown | fullcyclebioplastics.com |
Newlight, USA | PHB | Ocean microbes grown on greenhouse gas | Unknown | www.newlight.com |
Metabolix, USA | P3HB4HB | Escherichia coli | 5 000 | IP sold to CJ, Korea |
BOSK Bioproducts, Canada | PHAb | Forest wastes for PHA production | Unknown | www.bosk-bioproducts.com |
Genecis, Canada | PHBV | Unknown | Unknown | genecis.co |
TerraVerdae Bioworks,Canada | PHAb | Unknown | Unknown | terraverdae.com |
Kaneka, Japan | PHBHHx | Ralstonia eutropha | 5 000 | www.kaneka.be |
Nafigate, France | PHB | Toxic waste as substrates | Unknown | www.nafigate.com |
CJ, Korea | P3HB4HB | Escherichia coli | Unknown | www.cj.co.kr |
Helian Polymers, The Netherlands | PHB/PHBV | non-GMO bacteria | Unknown | helianpolymers.com |
Biocycle, Brazil | PHB | Bacillus spp. | 100 | fapesp.br |
Biomer, Germany | PHB | Alcaligenes latus | Unknown | biomer.de |
Bioextrax, Sweden | PHAb | Bioextrax DSP method | Unknown | bioextrax.com |
SABIO srl, Italy | PHAb | Organic wastes for PHA production | Unknown | www.bio-on.it |
Table 3 PHAs commercialization companies [6]
公司 Companies | PHA类型 PHAs Types | 技术 Technology | 规模(吨/年) Scale (ton/year) | 官方网站 Websites |
---|---|---|---|---|
Go!PHA, The Netherlands | All Types | PHA Global Promotion | Unknown | gopha.org |
PhaBuilder, China | All Types | Halomonas spp (NGIBa) | 1 000-10 000 | www.phabuilder.com |
Medpha, China | P3HB4HB | Halomonas spp (NGIBa) | 100 | www.medpha.com.cn |
COFCO, China | PHB | Halomonas spp (NGIBa) | 1 000 | www.cofco.com |
Bluepha, China | PHBHHx | Ralstonia eutropha and NGIB | 1 000 | www.bluepha.com |
TianAn Biopolymer, China | PHBV | Ralstonia eutropha | 2 000 | www.tianan-enmat.com |
GreenBio, Tianjin, China | P3HB4HB | Escherichia coli | 10000 | www.tjgreenbio.com |
Ecomann, Shenzhen, China | P3HB4HB | Escherichia coli | 10000 | ecomannbruce.plasway.com |
RWDC, Singapore and USA | PHBHHx | Ralstonia eutropha | Unknown | www.rwdc-industries.com |
Danimer Scientific, USA | PHBHHx | Ralstonia eutropha | 10 000 | danimerscientific.com |
Full Cycle, USA | PHAb | non-GMO bacteria | Unknown | fullcyclebioplastics.com |
Newlight, USA | PHB | Ocean microbes grown on greenhouse gas | Unknown | www.newlight.com |
Metabolix, USA | P3HB4HB | Escherichia coli | 5 000 | IP sold to CJ, Korea |
BOSK Bioproducts, Canada | PHAb | Forest wastes for PHA production | Unknown | www.bosk-bioproducts.com |
Genecis, Canada | PHBV | Unknown | Unknown | genecis.co |
TerraVerdae Bioworks,Canada | PHAb | Unknown | Unknown | terraverdae.com |
Kaneka, Japan | PHBHHx | Ralstonia eutropha | 5 000 | www.kaneka.be |
Nafigate, France | PHB | Toxic waste as substrates | Unknown | www.nafigate.com |
CJ, Korea | P3HB4HB | Escherichia coli | Unknown | www.cj.co.kr |
Helian Polymers, The Netherlands | PHB/PHBV | non-GMO bacteria | Unknown | helianpolymers.com |
Biocycle, Brazil | PHB | Bacillus spp. | 100 | fapesp.br |
Biomer, Germany | PHB | Alcaligenes latus | Unknown | biomer.de |
Bioextrax, Sweden | PHAb | Bioextrax DSP method | Unknown | bioextrax.com |
SABIO srl, Italy | PHAb | Organic wastes for PHA production | Unknown | www.bio-on.it |
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