Synthetic Biology Journal

   

Plant synthetic biology and bioproduction of human milk oligosaccharides

Wenwen YU1,2,3, Xueqin LV1,2, Zhaofeng LI3, Long LIU1,2   

  1. 1.Science Center for Future Foods，Jiangnan University，Wuxi 214122，Jiangsu，China
    2.School of Biotechnology，Key Laboratory of Sugar Chemistry and Biotechnology，Ministry of Education，Jiangnan University，Wuxi 214122，Jiangsu，China
    3.School of Food Science and Technology，Jiangnan University，Wuxi 214122，Jiangsu，China
  • Received:2024-12-02 Revised:2025-01-10 Published:2025-01-13
  • Contact: Wenwen YU, Long LIU

植物合成生物学与母乳低聚糖生物制造

于文文1,2,3, 吕雪芹1,2, 李兆丰3, 刘龙1,2   

  1. 1.江南大学未来食品科学中心，江苏 无锡 214112
    2.江南大学生物工程学院，糖化学与生物技术教育部重点实验室，江苏 无锡 214112
    3.江南大学食品学院，江苏 无锡 214112
  • 通讯作者: 于文文,刘龙
  • 作者简介:于文文（1994—），男，助理研究员。研究方向为乳基功能营养品的生物制造。E-mail：yuwenwen@stu.jiangnan.edu.cn
    刘龙（1980—），男，教授，博士生导师。主要从事合成生物学与食品生物制造的基础理论、关键技术与工程应用研究。以第一或通讯作者在Nature Chemical Biology等期刊发表论文200余篇；以第一完成人获国家科学技术进步奖二等奖等科技奖励7项。E-mail：longliu@jiangnan.edu.cn
  • 基金资助:
    国家自然科学基金(32400055);中国博士后科学基金面上资助(2024M761180);博士后创新人才支持计划(BX20240145);江苏省卓越博士后计划(2024ZB009)

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Abstract:

Human milk oligosaccharides (HMOs) are the third largest solid component in breast milk. They have broad applications due to their beneficial physiological functions such as regulating the immune system, maintaining digestive health, and promoting brain development. There is a growing interest in the development of green and efficient bioproduction of HMOs via synthetic biology technologies. Recently, Patrick M. Shih's team from the University of California, Berkeley, has engineered the model plant Nicotiana benthamiana as a photosynthetic platform for HMOs production. Specifically, the HMO biosynthetic enzymes were heterologously expressed in the cytosol to reconstruct the metabolic pathways required for HMOs bioproduction. Furthermore, they optimized the productions of HMOs by enhancing the supply of key precursors. Finally, several HMOs were successfully produced from cost-effective raw materials CO2. The reported study brings deeper insights into the green bioproduction of HMOs, and expand the potential application of plant synthetic biology technologies in the green and sustainable bioproduction of other dairy-based functional nutraceuticals.

Key words: human milk oligosaccharides, bioproduction, model plant, carbon dioxide, plant synthetic biology

摘要：

母乳低聚糖（Human milk oligosaccharides， HMOs）是母乳中仅次于乳糖和脂肪的第三大固体成分，具有调节免疫系统、维持消化健康及促进大脑发育等生理功能。近期，加州大学伯克利分校Patrick M. Shih基于合成生物学使能技术，在模式植物本生烟中重构HMOs代谢合成途径，利用太阳光能将CO2转化为系列HMOs。相关研究为HMOs的生物制造提供了新思路与新范例，扩宽了植物合成生物学在乳基功能营养品可持续发展领域中的应用前景。

关键词: 母乳低聚糖, 生物制造, 模式植物, 二氧化碳, 植物合成生物学

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