Synthetic Biology Journal ›› 2022, Vol. 3 ›› Issue (5): 953-965.DOI: 10.12211/2096-8280.2022-023
• Invited Review • Previous Articles Next Articles
Zhongliang SUN, Hui CHEN, Qiang WANG
Received:
2022-04-15
Revised:
2022-08-17
Online:
2022-11-16
Published:
2022-10-31
Contact:
Qiang WANG
孙中亮, 陈辉, 王强
通讯作者:
王强
作者简介:
基金资助:
CLC Number:
Zhongliang SUN, Hui CHEN, Qiang WANG. From CO2 to value-added products—carbon neutral microalgal green biomanufacturing[J]. Synthetic Biology Journal, 2022, 3(5): 953-965.
孙中亮, 陈辉, 王强. 从CO2到有机物——碳中和的微藻绿色生物制造[J]. 合成生物学, 2022, 3(5): 953-965.
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URL: https://synbioj.cip.com.cn/EN/10.12211/2096-8280.2022-023
表达产物 | 合成聚合物 | 表达株系 | 最终产率/[mg/(L·d)] | 参考文献 |
---|---|---|---|---|
1,3-丙二醇 | 聚酯、聚醚、聚氨酯、PTT | Synechococcus sp. PCC 7942 | 61 | [ |
Synechococcus sp. PCC 7942 | 8 | [ | ||
Synechococcus sp. PCC 7942 | 20.6 | [ | ||
Synechococcus sp. PCC 7120 | 2.3 | [ | ||
1,2-丙二醇 | 聚丙二醇 | Synechococcus sp. PCC 7942 | 15 | [ |
Synechocystis sp. PCC 6803 | 100 | [ | ||
异戊二烯 | 橡胶 | Synechocystis sp. PCC 6803 | 60 | [ |
Synechocystis sp. PCC 6803 | 1.7 | [ | ||
乙烯 | 聚乙烯、聚苯乙烯、PVC、聚酯 | Synechocystis sp. PCC 6803 | 2104 | [ |
Synechocystis sp. PCC 6803 | 19.2 | [ | ||
3-羟基丙酸 | 聚3-羟基丙酸 | Synechocystis sp. PCC 6803 | 139.5 | [ |
Synechococcus sp. PCC 7942 | 329.5 | [ | ||
2,3-丁二醇 | 树脂 | Synechococcus sp. PCC 7942 | 54.36 | [ |
Synechococcus sp. PCC 7942 | 300 | [ | ||
Synechococcus sp. PCC 7942 | 110 | [ | ||
Synechococcus sp. PCC 7942 | 100 | [ |
Tab. 1 Production of platform compound by cyanobacteria cell factory
表达产物 | 合成聚合物 | 表达株系 | 最终产率/[mg/(L·d)] | 参考文献 |
---|---|---|---|---|
1,3-丙二醇 | 聚酯、聚醚、聚氨酯、PTT | Synechococcus sp. PCC 7942 | 61 | [ |
Synechococcus sp. PCC 7942 | 8 | [ | ||
Synechococcus sp. PCC 7942 | 20.6 | [ | ||
Synechococcus sp. PCC 7120 | 2.3 | [ | ||
1,2-丙二醇 | 聚丙二醇 | Synechococcus sp. PCC 7942 | 15 | [ |
Synechocystis sp. PCC 6803 | 100 | [ | ||
异戊二烯 | 橡胶 | Synechocystis sp. PCC 6803 | 60 | [ |
Synechocystis sp. PCC 6803 | 1.7 | [ | ||
乙烯 | 聚乙烯、聚苯乙烯、PVC、聚酯 | Synechocystis sp. PCC 6803 | 2104 | [ |
Synechocystis sp. PCC 6803 | 19.2 | [ | ||
3-羟基丙酸 | 聚3-羟基丙酸 | Synechocystis sp. PCC 6803 | 139.5 | [ |
Synechococcus sp. PCC 7942 | 329.5 | [ | ||
2,3-丁二醇 | 树脂 | Synechococcus sp. PCC 7942 | 54.36 | [ |
Synechococcus sp. PCC 7942 | 300 | [ | ||
Synechococcus sp. PCC 7942 | 110 | [ | ||
Synechococcus sp. PCC 7942 | 100 | [ |
藻株 | 操作方法 | 有益结果 | 油脂含量或产率 | 参考文献 |
---|---|---|---|---|
Phaeodactylum tricornutum | 过表达内源性苹果酸脱氢酶 | 油脂含量增加250% | 57.8% | [ |
Chlamydomonas reinhardtii | Lobosphaera incise中的GPAT基因(LiGPAT)在莱茵衣藻中过表达 | TAG含量比野生型增加50% | 50.0% | [ |
Thalassiosira pseudonana | 转化反义结构体,阻止脂质分解代谢 | 油脂含量比野生型增加230% | 18.8% | [ |
Phaeodactylum tricornutum | 过表达Ⅱ型甘油二酯酰基转移酶(DGAT2D)基因 | TAG含量增加35% | 37.2% | [ |
Chlorella spp | 拟南芥激酶的上调和过表达 | 油脂产量增加110.4% | 27.5% | [ |
Nannochloropsis | 过表达新型bZIP1转录因子NobZIP1N | 油脂含量增加而不影响微藻生长 | 40% | [ |
Chlamydomonas reinhardtii | 使用CRISPR-Cas9敲除磷脂酶A2基因 | 油脂含量可达64.25% | 80.92 mg/(L·d) | [ |
Chlamydomonas reinhardtii | 使用CRISPR-Cas9 RNP方法产生葡萄糖焦磷酸化酶基因(AGP)突变的突变体 | 油脂含量比野生型增加274% | 57.67% | [ |
Nannochloropsis | 通过插入突变产生突变体Mut68 | 脂肪酸甲酯含量和产量分别比野生型增加34%和75% | 78.3 mg/(L·d) | [ |
Planktochlorella nurekis | 利用细胞松弛素B和秋水仙碱调控DNA水平 | 油脂含量比野生型增加10%~60% | 12%~26% | [ |
Tab. 2 Genetic engineering and metabolic engineering methods are used to improve the yield of microalgae lipid
藻株 | 操作方法 | 有益结果 | 油脂含量或产率 | 参考文献 |
---|---|---|---|---|
Phaeodactylum tricornutum | 过表达内源性苹果酸脱氢酶 | 油脂含量增加250% | 57.8% | [ |
Chlamydomonas reinhardtii | Lobosphaera incise中的GPAT基因(LiGPAT)在莱茵衣藻中过表达 | TAG含量比野生型增加50% | 50.0% | [ |
Thalassiosira pseudonana | 转化反义结构体,阻止脂质分解代谢 | 油脂含量比野生型增加230% | 18.8% | [ |
Phaeodactylum tricornutum | 过表达Ⅱ型甘油二酯酰基转移酶(DGAT2D)基因 | TAG含量增加35% | 37.2% | [ |
Chlorella spp | 拟南芥激酶的上调和过表达 | 油脂产量增加110.4% | 27.5% | [ |
Nannochloropsis | 过表达新型bZIP1转录因子NobZIP1N | 油脂含量增加而不影响微藻生长 | 40% | [ |
Chlamydomonas reinhardtii | 使用CRISPR-Cas9敲除磷脂酶A2基因 | 油脂含量可达64.25% | 80.92 mg/(L·d) | [ |
Chlamydomonas reinhardtii | 使用CRISPR-Cas9 RNP方法产生葡萄糖焦磷酸化酶基因(AGP)突变的突变体 | 油脂含量比野生型增加274% | 57.67% | [ |
Nannochloropsis | 通过插入突变产生突变体Mut68 | 脂肪酸甲酯含量和产量分别比野生型增加34%和75% | 78.3 mg/(L·d) | [ |
Planktochlorella nurekis | 利用细胞松弛素B和秋水仙碱调控DNA水平 | 油脂含量比野生型增加10%~60% | 12%~26% | [ |
藻株 | 操作方法 | 有益结果 | 氢气产量或产率 | 参考文献 |
---|---|---|---|---|
Chlamydomonas reinhardtii | 设计光诱导系统,利用该系统设计蓝光诱导产氢转基因藻类 | 成功激活了人工miRNA,提高微藻的产氢能力 | 20 μL/(L·h) | [ |
Chlamydomonas reinhardtii | 将大肠杆菌的丙酮酸氧化酶和过氧化氢酶基因整合到衣藻叶绿体基因组中 | 生物氢产量增加了2倍 | 1.04 μmol/(L·h) | [ |
Chlorella sp. DT. | 敲除psbO基因 | 生物氢产量比野生型提高了9倍 | 350 mL/L | [ |
Chlamydomonas reinhardtii | 截短捕光天线 | 氢气产量比野生型增加6倍 | 30 mL/L | [ |
Synechocystis sp. PCC 6803 | 添加抑制光合和呼吸作用电子传递链的抑制剂 | 氢气产量增加了30倍 | 1.25 μmol/(L·h) | [ |
Chlamydomonas reinhardtii | 热诱导人工miRNA表达系统 | 氢气合成增加60% | 90 μL/mg Chl | [ |
Tab. 3 Various genetic engineering methods for improving hydrogen production rate of microalgae
藻株 | 操作方法 | 有益结果 | 氢气产量或产率 | 参考文献 |
---|---|---|---|---|
Chlamydomonas reinhardtii | 设计光诱导系统,利用该系统设计蓝光诱导产氢转基因藻类 | 成功激活了人工miRNA,提高微藻的产氢能力 | 20 μL/(L·h) | [ |
Chlamydomonas reinhardtii | 将大肠杆菌的丙酮酸氧化酶和过氧化氢酶基因整合到衣藻叶绿体基因组中 | 生物氢产量增加了2倍 | 1.04 μmol/(L·h) | [ |
Chlorella sp. DT. | 敲除psbO基因 | 生物氢产量比野生型提高了9倍 | 350 mL/L | [ |
Chlamydomonas reinhardtii | 截短捕光天线 | 氢气产量比野生型增加6倍 | 30 mL/L | [ |
Synechocystis sp. PCC 6803 | 添加抑制光合和呼吸作用电子传递链的抑制剂 | 氢气产量增加了30倍 | 1.25 μmol/(L·h) | [ |
Chlamydomonas reinhardtii | 热诱导人工miRNA表达系统 | 氢气合成增加60% | 90 μL/mg Chl | [ |
分类 | 藻株 | 合成产物 | 参考文献 |
---|---|---|---|
原核藻 | Synechocystis sp. PCC 6803 | 对香豆酸 | [ |
Synechocystis sp. PCC 6803 | 叶黄素 | [ | |
Synechococcus elongatus UTEX 2973 | 柠檬烯 | [ | |
Synechocystis sp. PCC 6803 | 乳酸 | [ | |
Synechococcus sp. PCC 7002 | 虾青素 | [ | |
Anabaena sp. PCC7120 | 法尼烯 | [ | |
Synechococcus sp. PCC 7942 | 柠檬烯 | [ | |
真核藻 | Chlamydomonas reinhardtii | 类异戊二烯 | [ |
Chlamydomonas reinhardtii | 木糖醇 | [ | |
Chlamydomonas reinhardtii | 倍半萜 | [ | |
Phaeodactylum tricornutum | DHA | [ | |
Bacillariophyta | 没药烯 | [ | |
Nannochloropsis | EPA | [ | |
Phaeodactylum tricornutum | 岩藻黄质 | [ |
Tab. 4 Biosynthesis of high value-added compounds by microalgae chassis cells
分类 | 藻株 | 合成产物 | 参考文献 |
---|---|---|---|
原核藻 | Synechocystis sp. PCC 6803 | 对香豆酸 | [ |
Synechocystis sp. PCC 6803 | 叶黄素 | [ | |
Synechococcus elongatus UTEX 2973 | 柠檬烯 | [ | |
Synechocystis sp. PCC 6803 | 乳酸 | [ | |
Synechococcus sp. PCC 7002 | 虾青素 | [ | |
Anabaena sp. PCC7120 | 法尼烯 | [ | |
Synechococcus sp. PCC 7942 | 柠檬烯 | [ | |
真核藻 | Chlamydomonas reinhardtii | 类异戊二烯 | [ |
Chlamydomonas reinhardtii | 木糖醇 | [ | |
Chlamydomonas reinhardtii | 倍半萜 | [ | |
Phaeodactylum tricornutum | DHA | [ | |
Bacillariophyta | 没药烯 | [ | |
Nannochloropsis | EPA | [ | |
Phaeodactylum tricornutum | 岩藻黄质 | [ |
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