Synthetic Biology Journal ›› 2023, Vol. 4 ›› Issue (4): 720-737.DOI: 10.12211/2096-8280.2022-053
• Invited Review • Previous Articles Next Articles
Yannan WANG, Yuhui SUN
Received:
2022-09-28
Revised:
2022-11-13
Online:
2023-09-14
Published:
2023-08-31
Contact:
Yuhui SUN
王雁南, 孙宇辉
通讯作者:
孙宇辉
作者简介:
基金资助:
CLC Number:
Yannan WANG, Yuhui SUN. Base editing technology and its application in microbial synthetic biology[J]. Synthetic Biology Journal, 2023, 4(4): 720-737.
王雁南, 孙宇辉. 碱基编辑技术及其在微生物合成生物学中的应用[J]. 合成生物学, 2023, 4(4): 720-737.
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URL: https://synbioj.cip.com.cn/EN/10.12211/2096-8280.2022-053
碱基 编辑器 | 版本 | 组成 | PAM (5′-3′) | 特点 | 参考 文献 |
---|---|---|---|---|---|
CBE | BE3 | rAPOBEC1-SpnCas9-UGI | NGG | 初代碱基编辑器,TC基序偏好性 | [ |
Target-AID | SpnCas9-CDA1-UGI | NGG | 初代碱基编辑器 | [ | |
hA3A-BE3 | hAPOBEC3A-SpnCas9-UGI | NGG | 可编辑甲基化的C及GC基序的C | [ | |
BE4-Max | rAPOBEC1-SpnCas9-UGI-UGI | NGG | 密码子优化,高活性 | [ | |
dCpf1-BE | rAPOBEC1-dLbCpf1-UGI | TTTV | 识别富含T的PAM | [ | |
Target-AID-NG | SpnCas9 (NG)-CDA1-UGI | NG | 识别NG PAM | [ | |
A3A-PBE | hAPOBEC3A-SpnCas9-UGI | NGG | 1~17号位编辑窗口,几乎无基序偏好性 | [ | |
BE-PLUS | SunTag-SpnCas9-scFv-rAPOBEC1-UGI | NGG | 4~16号位编辑窗口,高保真 | [ | |
A3G-BEs | hAPOBEC3G-SpnCas9-UGI-UGI | NGG | 编辑CC基序中的第二个C | [ | |
BE-PAPAPAP | rAPOBEC1-SpnCas9-UGI | NGG | 5~6号位编辑窗口,PAPAPAP替换XTEN linker | [ | |
HF-BE3 | rAPOBEC1-HFnCas9-UGI | NGG | 减少sgRNA依赖的脱靶编辑 | [ | |
YE1-BE4 | rAPOBEC1 (W90Y, R126E)-SpnCas9-UGI-UGI | NGG | 缩小编辑窗口,减少非sgRNA依赖的脱靶编辑 | [ | |
SECURE BE3 | rAPOBEC1 (R33A, K34A)-SpnCas9-UGI | NGG | 减少RNA层面的脱靶编辑,缩小编辑窗口 | [ | |
DdCBEs | TALE-split DddAtox-UGI | None | 同时编辑DNA双链,可编辑线粒体、叶绿体基因组 | [ | |
ZFDs | ZFP-split DddAtox-UGI | None | 同时编辑DNA双链,可编辑线粒体、叶绿体基因组 | [ | |
ABE | ABE7.10 | ecTadA-ecTadA*-SpnCas9 | NGG | 初代碱基编辑器 | [ |
ABEmax | ecTadA-ecTadA*-SpnCas9 | NGG | 密码子优化,高活性 | [ | |
ABE8e | ecTadA-ecTadA*-SpnCas9 | NGG | 高活性,TadA-8e | [ | |
TaC9-ABE | ecTadA-ecTadA*-TALE,SpnCas9 | NGG | 消除sgRNA依赖的脱靶编辑 | [ | |
ABE-nSpCas9-DS | ecTadA*-ecTadA*-SpnCas9 (DS) | NGG | 拓宽编辑窗口,减少RNA层面的脱靶编辑 | [ | |
ACBE | A&C-BEmax | rAPOBEC1/hAID-ecTadA-ecTadA*-SpnCas9-UGI-UGI | NGG | 可同时编辑A或C | [ |
sgBE | SpnCas9, MCP-cytosine/adenosine deaminase | NGG | 可同时编辑A或C | [ | |
GBE | CGBE1 | eUNG-rAPOBEC1 (R33A)-SpnCas9-UGI-UGI | NGG | 可实现C变G的碱基颠换 | [ |
CGBE | rAPOBEC1-SpnCas9-rXRCC1 | NGG | 利用碱基切除修复通路实现C变G的碱基颠换 | [ |
Table 1 Comparison of representative base editors
碱基 编辑器 | 版本 | 组成 | PAM (5′-3′) | 特点 | 参考 文献 |
---|---|---|---|---|---|
CBE | BE3 | rAPOBEC1-SpnCas9-UGI | NGG | 初代碱基编辑器,TC基序偏好性 | [ |
Target-AID | SpnCas9-CDA1-UGI | NGG | 初代碱基编辑器 | [ | |
hA3A-BE3 | hAPOBEC3A-SpnCas9-UGI | NGG | 可编辑甲基化的C及GC基序的C | [ | |
BE4-Max | rAPOBEC1-SpnCas9-UGI-UGI | NGG | 密码子优化,高活性 | [ | |
dCpf1-BE | rAPOBEC1-dLbCpf1-UGI | TTTV | 识别富含T的PAM | [ | |
Target-AID-NG | SpnCas9 (NG)-CDA1-UGI | NG | 识别NG PAM | [ | |
A3A-PBE | hAPOBEC3A-SpnCas9-UGI | NGG | 1~17号位编辑窗口,几乎无基序偏好性 | [ | |
BE-PLUS | SunTag-SpnCas9-scFv-rAPOBEC1-UGI | NGG | 4~16号位编辑窗口,高保真 | [ | |
A3G-BEs | hAPOBEC3G-SpnCas9-UGI-UGI | NGG | 编辑CC基序中的第二个C | [ | |
BE-PAPAPAP | rAPOBEC1-SpnCas9-UGI | NGG | 5~6号位编辑窗口,PAPAPAP替换XTEN linker | [ | |
HF-BE3 | rAPOBEC1-HFnCas9-UGI | NGG | 减少sgRNA依赖的脱靶编辑 | [ | |
YE1-BE4 | rAPOBEC1 (W90Y, R126E)-SpnCas9-UGI-UGI | NGG | 缩小编辑窗口,减少非sgRNA依赖的脱靶编辑 | [ | |
SECURE BE3 | rAPOBEC1 (R33A, K34A)-SpnCas9-UGI | NGG | 减少RNA层面的脱靶编辑,缩小编辑窗口 | [ | |
DdCBEs | TALE-split DddAtox-UGI | None | 同时编辑DNA双链,可编辑线粒体、叶绿体基因组 | [ | |
ZFDs | ZFP-split DddAtox-UGI | None | 同时编辑DNA双链,可编辑线粒体、叶绿体基因组 | [ | |
ABE | ABE7.10 | ecTadA-ecTadA*-SpnCas9 | NGG | 初代碱基编辑器 | [ |
ABEmax | ecTadA-ecTadA*-SpnCas9 | NGG | 密码子优化,高活性 | [ | |
ABE8e | ecTadA-ecTadA*-SpnCas9 | NGG | 高活性,TadA-8e | [ | |
TaC9-ABE | ecTadA-ecTadA*-TALE,SpnCas9 | NGG | 消除sgRNA依赖的脱靶编辑 | [ | |
ABE-nSpCas9-DS | ecTadA*-ecTadA*-SpnCas9 (DS) | NGG | 拓宽编辑窗口,减少RNA层面的脱靶编辑 | [ | |
ACBE | A&C-BEmax | rAPOBEC1/hAID-ecTadA-ecTadA*-SpnCas9-UGI-UGI | NGG | 可同时编辑A或C | [ |
sgBE | SpnCas9, MCP-cytosine/adenosine deaminase | NGG | 可同时编辑A或C | [ | |
GBE | CGBE1 | eUNG-rAPOBEC1 (R33A)-SpnCas9-UGI-UGI | NGG | 可实现C变G的碱基颠换 | [ |
CGBE | rAPOBEC1-SpnCas9-rXRCC1 | NGG | 利用碱基切除修复通路实现C变G的碱基颠换 | [ |
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